FastQCFastQC Report
Thu 26 May 2016
SRR1512616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50950.24351262298701806No Hit
CTGTAGGACGTGGAATATGGCAAGA48060.22970003259580155No Hit
GTCCTAAAGTGTGTATTTCTCATTT46800.2236779343629528No Hit
CTTTAGGACGTGAAATATGGCGAGG37990.18157104116343115No Hit
GTATCAACGCAGAGTACTTTTTTTT37560.17951588065539548No Hit
GTACATGGGAAGCAGTGGTATCAAC26370.1260339130160484No Hit
GTCCTACAGTGTGCATTTCTCATTT26240.12541258542059577No Hit
GGTATCAACGCAGAGTACTTTTTTT23100.11040513426889338No Hit
CTGAAGGACCTGGAATATGGCGAGA23030.11007057325595733No Hit
TATCAACGCAGAGTACTTTTTTTTT21770.1040484750231086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8500.012.7061084
GGCGAGG11500.011.93411119
GGACGTT907.69635E-611.5791536
GCGCCAC855.466841E-511.14758413
GACCGTG600.005998582611.0528277
GTCCTAT3300.010.9542821
GTCCTAC24750.010.7919961
GTCCTAA14450.010.5626071
TTAGGAC17150.010.4963173
TGAGTTA8100.010.40854212
AGGACCT18450.010.3724565
AGGACGT30450.010.3605675
TCCAACG2200.010.32419918
TAGAAAT9600.010.263344
TCCTACA27450.010.2511362
GTATAAG2750.010.2239961
GTCCTAG3550.010.1828541
TGGCGAG28050.010.15550318
AAATGTC9350.010.13246257
GGACGTG29750.010.1266736
Produced by FastQC (version 0.11.5)