FastQCFastQC Report
Thu 26 May 2016
SRR1512616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57460.2746267971900698No Hit
CTGTAGGACGTGGAATATGGCAAGA55050.2631083394589862No Hit
GTCCTAAAGTGTGTATTTCTCATTT50900.24327365083492092No Hit
GTATCAACGCAGAGTACTTTTTTTT46870.22401249537588888No Hit
CTTTAGGACGTGAAATATGGCGAGG45150.21579185334374612No Hit
GGTATCAACGCAGAGTACTTTTTTT31570.15088701683415429No Hit
TATCAACGCAGAGTACTTTTTTTTT30230.14448256315795008No Hit
GTACATGGGAAGCAGTGGTATCAAC29920.1430009358149476No Hit
GTCCTACAGTGTGCATTTCTCATTT28110.13435014390903No Hit
CTGAAGGACCTGGAATATGGCGAGA27950.13358543302231904No Hit
CCCATGTACTCTGCGTTGATACCAC21580.10314038084513935No Hit
CTGTAGGACCTGGAATATGGCGAGA21420.1023756699584284No Hit
ATTTAGAAATGTCCACTGTAGGACG20960.10017712615913443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2600.013.88103518
CCAACGA1900.012.99664719
TAGGACC8900.011.5303544
AAATGTC7700.011.1031567
AAGTGCG600.00587972711.0825947
GGCGAGG12500.010.94117719
AGGACGA1053.4583427E-610.8595345
CCACCTT7500.010.76517713
TGTAGAA9200.010.7412392
ACCTTTT8150.010.72141815
TGCCGTG803.7741743E-410.6867887
AATGTCC8300.010.6438688
CACCTTT8300.010.52765714
GTAGAAC8600.010.4962463
AGGACCT19300.010.4375315
CCAACGT1201.5292244E-610.28901319
GGACCTG19250.010.2151476
TGGCGAG27600.010.18577918
AGGACGT28750.010.1465825
GGACGTG28400.010.1351296
Produced by FastQC (version 0.11.5)