FastQCFastQC Report
Thu 26 May 2016
SRR1512615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898966
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55210.29073716959650675No Hit
GTATCAACGCAGAGTACTTTTTTTT53720.28289079425329366No Hit
GTCCTAAAGTGTGTATTTCTCATTT51280.27004169637581715No Hit
CTGTAGGACGTGGAATATGGCAAGA49980.26319586553945673No Hit
CTTTAGGACGTGAAATATGGCGAGG47050.2477664160390444No Hit
GGTATCAACGCAGAGTACTTTTTTT34380.18104589550313172No Hit
TATCAACGCAGAGTACTTTTTTTTT33470.1762538139176794No Hit
GTCCTACAGTGTGCATTTCTCATTT30510.16066638370565875No Hit
CTGAAGGACCTGGAATATGGCGAGA22150.11664242540414099No Hit
CTGTAGGACCTGGAATATGGCGAGA22060.11616848326931603No Hit
ATTTAGAAATGTCCACTGTAGGACG20080.1057417563031671No Hit
TTTCTAAATTTTCCACCTTTTTCAG19640.10342470586624511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2200.014.62366618
TAGGACC8950.013.1261934
GTTGGAC957.65267E-812.9653083
AGGCGAG604.2320642E-412.61649718
CCAACGT1501.4551915E-1111.98693919
GCCGGTT1603.6379788E-1211.84017711
CCAACGA1302.7794158E-911.64722919
CCGCAGA957.356546E-611.6259911
CGGTTTC1657.2759576E-1211.4871613
CTATAGG3500.011.4750031
ACCTTTT7800.011.18114815
ATACGGC855.441474E-511.15255115
AGGACCT17750.011.1020985
ATAGGAC3500.011.0988743
CACCTTT7800.011.05961314
CCGGTTT1803.6379788E-1211.05200112
ACCGTTC600.006106393411.0263148
GTCCTAT2550.011.0250041
GGTATCA10750.011.0213411
GGACCTG17200.011.0167426
Produced by FastQC (version 0.11.5)