##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1898966 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.458751236199067 31.0 26.0 33.0 10.0 34.0 2 28.13054683443516 31.0 27.0 34.0 16.0 34.0 3 29.297693586930993 31.0 28.0 34.0 23.0 34.0 4 33.10709038497793 35.0 35.0 37.0 27.0 37.0 5 33.41938770888999 37.0 35.0 37.0 28.0 37.0 6 33.63201394864363 37.0 35.0 37.0 29.0 37.0 7 33.607311557974185 37.0 35.0 37.0 29.0 37.0 8 33.627562052190505 37.0 35.0 37.0 29.0 37.0 9 35.136207283332084 39.0 35.0 39.0 29.0 39.0 10 35.04900824975276 39.0 35.0 39.0 28.0 39.0 11 35.09741985901801 39.0 35.0 39.0 29.0 39.0 12 35.032489786546996 39.0 35.0 39.0 28.0 39.0 13 35.01095227613343 39.0 35.0 39.0 28.0 39.0 14 36.12995703977849 40.0 37.0 41.0 27.0 41.0 15 36.06288685526755 40.0 37.0 41.0 27.0 41.0 16 35.926289886180165 40.0 36.0 41.0 27.0 41.0 17 35.881414938445445 40.0 36.0 41.0 27.0 41.0 18 35.846657602084505 40.0 36.0 41.0 27.0 41.0 19 35.86986391541502 40.0 37.0 41.0 27.0 41.0 20 35.67901215714236 40.0 36.0 41.0 26.0 41.0 21 35.663041360403504 40.0 36.0 41.0 27.0 41.0 22 35.575992408500205 40.0 36.0 41.0 26.0 41.0 23 35.42280167206785 40.0 36.0 41.0 25.0 41.0 24 35.31362752150381 40.0 36.0 41.0 25.0 41.0 25 35.1971388639923 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1952560748348482 1101 2 -1.2798098664844773 1101 3 -2.1084331491362462 1101 4 -2.5032042051236765 1101 5 -2.519201223266215 1101 6 -2.610599788606468 1101 7 -2.6570034305272543 1101 8 -2.6958466398926184 1101 9 -2.838543485230204 1101 10 -3.3644793759096103 1101 11 -3.1060025650712184 1101 12 -2.755401545712374 1101 13 -3.2467405025609004 1101 14 -3.7235940889300636 1101 15 -3.792884469501381 1101 16 -3.998923082467396 1101 17 -3.9955305521173834 1101 18 -4.242328971212469 1101 19 -4.10365713300439 1101 20 -4.193203268972066 1101 21 -4.553170097846355 1101 22 -4.782647404252572 1101 23 -5.023212627263984 1101 24 -4.820032476812994 1101 25 -5.465390383526074 1102 1 1.0972359659731694 1102 2 0.3661239498361546 1102 3 -0.24330947004852632 1102 4 -0.799186297875373 1102 5 -0.5687583362538788 1102 6 -0.5742694514593367 1102 7 -0.5305477976451698 1102 8 -0.5593090745856415 1102 9 -0.8120602021058474 1102 10 -0.9010520243196822 1102 11 -0.8913842833553858 1102 12 -0.861526446019127 1102 13 -0.8966189381220886 1102 14 -1.3058379506877742 1102 15 -1.1997525815621586 1102 16 -1.4853923026190756 1102 17 -1.1445181593085252 1102 18 -1.4074566640450357 1102 19 -1.4624316623742288 1102 20 -1.2885687315896064 1102 21 -1.3922215310134192 1102 22 -1.451798504650455 1102 23 -1.4058432950990962 1102 24 -1.6261188005720584 1102 25 -1.465531607881772 1103 1 2.5874239474831846 1103 2 0.9330540987832521 1103 3 0.2786080954368373 1103 4 -0.02508408934943418 1103 5 -0.23002900908387147 1103 6 -0.28830745864988216 1103 7 -0.4935757894273962 1103 8 -0.4111056847551353 1103 9 -0.4576621538264405 1103 10 -0.7713766262714046 1103 11 -0.7784374420610902 1103 12 -0.7496540782738847 1103 13 -0.5361268990876766 1103 14 -0.7763972727216668 1103 15 -0.9237954680542018 1103 16 -1.0736010853617515 1103 17 -1.0696347489335878 1103 18 -1.1223796224425726 1103 19 -1.0861332545673434 1103 20 -1.0476750490010147 1103 21 -1.2500340631140858 1103 22 -1.1798680988980124 1103 23 -1.2284593711134804 1103 24 -1.2513334898375916 1103 25 -1.3107398770137735 1104 1 2.0809811250987558 1104 2 1.178628308028994 1104 3 0.0586433752421911 1104 4 -0.49272642511586184 1104 5 -0.6445740212493192 1104 6 -0.8216018625428276 1104 7 -0.9092655704492927 1104 8 -0.9224828137089105 1104 9 -1.028146338741287 1104 10 -0.9451333172689758 1104 11 -1.007506785126779 1104 12 -1.0580518884928622 1104 13 -1.224238821228795 1104 14 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1107 12 0.08313899978208639 1107 13 0.058601623249096235 1107 14 0.033769148485987444 1107 15 -0.07175005813765978 1107 16 -0.13314084972110862 1107 17 0.03254590889655162 1107 18 -0.11069331905523683 1107 19 -0.27737674990362393 1107 20 -0.19662939362545728 1107 21 -0.12198742590249623 1107 22 -0.13997781907474405 1107 23 -0.24226472211985595 1107 24 -0.20174929739773262 1107 25 -0.2745319732337066 1108 1 -3.1746634561157876 1108 2 -4.732064153144769 1108 3 -4.312637405848509 1108 4 -3.005544243591082 1108 5 -1.8424248959733305 1108 6 -1.2369347080072117 1108 7 -1.0394665349286853 1108 8 -0.7510131397679913 1108 9 -0.8780503286336412 1108 10 -0.6530887085336161 1108 11 -0.7369271592847397 1108 12 -0.6911669317443199 1108 13 -0.6116693155401123 1108 14 -1.0129705375031648 1108 15 -0.9099124346351743 1108 16 -0.9188517280352784 1108 17 -0.7468224096019682 1108 18 -0.8296161262986104 1108 19 -0.6614828689632333 1108 20 -0.6773408587696466 1108 21 -0.7523296929341328 1108 22 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0.3654237505322868 2316 22 0.5252261784314101 2316 23 0.4786730573531415 2316 24 0.35108543899585953 2316 25 0.4734453761043511 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78366.0 3 17.0 4 728.0 5 2889.0 6 3380.0 7 3161.0 8 2774.0 9 2614.0 10 2549.0 11 2499.0 12 2578.0 13 2683.0 14 2772.0 15 2892.0 16 2904.0 17 3115.0 18 3509.0 19 4286.0 20 5402.0 21 6634.0 22 8068.0 23 9422.0 24 10896.0 25 12419.0 26 14222.0 27 16661.0 28 19475.0 29 22935.0 30 28500.0 31 36774.0 32 48277.0 33 65261.0 34 92610.0 35 137885.0 36 222474.0 37 289747.0 38 727588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72510762394112 21.066126568442556 12.62599056582643 24.582775241789896 2 13.771644850304076 20.574930286852975 43.54776063006753 22.105664232775418 3 18.265967994098816 25.69125475606172 30.429666744582274 25.613110505257193 4 11.811162792660845 17.573843179794107 39.91309339400053 30.701900633544515 5 12.329832175452792 38.637148844660096 36.76927856596174 12.26374041392537 6 30.230270483645068 38.5303303284286 18.85448429523004 12.384914892696298 7 26.65506276575383 32.08302358461136 23.523278475645025 17.738635173989778 8 23.541466944495063 39.02760061375779 21.362732436993433 16.06820000475372 9 26.290165479266918 15.185289793289497 21.37155889441161 37.15298583303198 10 14.858946643979346 29.305594845530585 35.83903683030531 19.996421680184763 11 33.04586463586155 22.340070138773445 26.659330858157652 17.954734367207354 12 23.356007993134263 24.925288684943624 33.22740213635831 18.4913011855638 13 28.88955402258076 23.0430288710253 26.20441986617088 21.862997240223063 14 19.845989453878136 23.72534258001981 28.54131338707055 27.887354579031502 15 21.76947524430742 33.49617852736586 23.7373553243034 20.996990904023313 16 20.495325168939033 28.536923676972798 30.406062277100766 20.561688876987404 17 20.448966760413107 29.885514322882212 29.259420776144303 20.406098140560385 18 20.31058277149605 27.66181573496233 32.42674125578879 19.600860237752826 19 23.109727851460566 27.051824400826863 28.49872478186225 21.33972296585032 20 23.074950764216386 28.261207900305518 29.37174981838287 19.292091517095223 21 21.812031840764515 27.215041252100864 28.245248818635872 22.727678088498752 22 22.490673242701632 29.629226743832987 28.211084385701348 19.669015627764033 23 20.87018322146022 29.239298011609762 29.333531015109994 20.556987751820028 24 22.65267094920125 27.389326768658606 30.055944129828415 19.902058152311724 25 21.73676600224504 28.45909357077475 29.4425665320066 20.361573894973606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1636.0 1 1636.0 2 1617.5 3 1599.0 4 1599.0 5 1599.0 6 2632.5 7 3666.0 8 3666.0 9 3666.0 10 4970.5 11 6275.0 12 6275.0 13 6275.0 14 9599.5 15 12924.0 16 12924.0 17 12924.0 18 21722.5 19 30521.0 20 30521.0 21 30521.0 22 47071.0 23 63621.0 24 63621.0 25 63621.0 26 91279.5 27 118938.0 28 118938.0 29 118938.0 30 150485.5 31 182033.0 32 182033.0 33 182033.0 34 198339.5 35 214646.0 36 214646.0 37 214646.0 38 222663.0 39 230680.0 40 230680.0 41 230680.0 42 235383.5 43 240087.0 44 240087.0 45 240087.0 46 238689.5 47 237292.0 48 237292.0 49 237292.0 50 219022.5 51 200753.0 52 200753.0 53 200753.0 54 171839.5 55 142926.0 56 142926.0 57 142926.0 58 119680.0 59 96434.0 60 96434.0 61 96434.0 62 77271.5 63 58109.0 64 58109.0 65 58109.0 66 44302.5 67 30496.0 68 30496.0 69 30496.0 70 22081.5 71 13667.0 72 13667.0 73 13667.0 74 9697.5 75 5728.0 76 5728.0 77 5728.0 78 4115.0 79 2502.0 80 2502.0 81 2502.0 82 1900.0 83 1298.0 84 1298.0 85 1298.0 86 1139.0 87 980.0 88 980.0 89 980.0 90 907.5 91 835.0 92 835.0 93 835.0 94 770.0 95 705.0 96 705.0 97 705.0 98 660.0 99 615.0 100 615.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.956820711903215 2 0.21369524256885064 3 0.19763387022200501 4 0.1894715334555753 5 0.16414195936104173 6 0.21401120399206727 7 0.4027454941268037 8 0.3007426146650335 9 0.2512419916944274 10 0.2223315214701053 11 0.21859264462870845 12 0.22854542946003248 13 0.24276369350478105 14 0.2532957409453355 15 0.33549837121886333 16 0.49390036472480287 17 0.4987977667846607 18 0.21806604225668075 19 0.18978749487879193 20 0.18404752902368973 21 0.2059541877000431 22 0.274939098435675 23 0.19294710911095828 24 0.31627738463985133 25 0.35777365155563606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1898966.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.43715092158945 #Duplication Level Percentage of deduplicated Percentage of total 1 84.8177450113212 60.591380512026426 2 10.448384078904615 14.928055806628828 3 2.6257082852757967 5.627193571539448 4 0.9010949957608358 2.5748663682742334 5 0.3959683442699806 1.4143425184893244 6 0.2019120941224529 0.8654414844431906 7 0.1255974797968238 0.6280628283731889 8 0.08599653950925691 0.49146782173257725 9 0.06049442969924468 0.3889394733906382 >10 0.29026288351948115 3.7221725016151965 >50 0.02253502191261681 1.1465313758414484 >100 0.019694630070167227 2.905190350228787 >500 0.0025259838464091557 1.3187644029317522 >1k 0.0018573410635361407 2.547304261232788 >5k 2.228809276243369E-4 0.8502867232521562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5521 0.29073716959650675 No Hit GTATCAACGCAGAGTACTTTTTTTT 5372 0.28289079425329366 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5128 0.27004169637581715 No Hit CTGTAGGACGTGGAATATGGCAAGA 4998 0.26319586553945673 No Hit CTTTAGGACGTGAAATATGGCGAGG 4705 0.2477664160390444 No Hit GGTATCAACGCAGAGTACTTTTTTT 3438 0.18104589550313172 No Hit TATCAACGCAGAGTACTTTTTTTTT 3347 0.1762538139176794 No Hit GTCCTACAGTGTGCATTTCTCATTT 3051 0.16066638370565875 No Hit CTGAAGGACCTGGAATATGGCGAGA 2215 0.11664242540414099 No Hit CTGTAGGACCTGGAATATGGCGAGA 2206 0.11616848326931603 No Hit ATTTAGAAATGTCCACTGTAGGACG 2008 0.1057417563031671 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1964 0.10342470586624511 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.266023720277245E-5 0.0 3 0.0 0.0 0.0 5.266023720277245E-5 0.0 4 0.0 0.0 0.0 1.053204744055449E-4 0.0 5 0.0 0.0 0.0 1.053204744055449E-4 0.0 6 1.053204744055449E-4 0.0 0.0 1.053204744055449E-4 0.0 7 1.053204744055449E-4 0.0 0.0 1.053204744055449E-4 0.0 8 1.053204744055449E-4 0.0 0.0 1.053204744055449E-4 0.0 9 1.5798071160831736E-4 0.0 0.0 1.053204744055449E-4 0.0 10 1.5798071160831736E-4 0.0 0.0 1.053204744055449E-4 0.0 11 1.5798071160831736E-4 0.0 0.0 1.053204744055449E-4 0.0 12 1.5798071160831736E-4 0.0 0.0 1.053204744055449E-4 0.0 13 1.5798071160831736E-4 0.0 0.0 1.053204744055449E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 220 0.0 14.623666 18 TAGGACC 895 0.0 13.126193 4 GTTGGAC 95 7.65267E-8 12.965308 3 AGGCGAG 60 4.2320642E-4 12.616497 18 CCAACGT 150 1.4551915E-11 11.986939 19 GCCGGTT 160 3.6379788E-12 11.840177 11 CCAACGA 130 2.7794158E-9 11.647229 19 CCGCAGA 95 7.356546E-6 11.625991 1 CGGTTTC 165 7.2759576E-12 11.48716 13 CTATAGG 350 0.0 11.475003 1 ACCTTTT 780 0.0 11.181148 15 ATACGGC 85 5.441474E-5 11.152551 15 AGGACCT 1775 0.0 11.102098 5 ATAGGAC 350 0.0 11.098874 3 CACCTTT 780 0.0 11.059613 14 CCGGTTT 180 3.6379788E-12 11.052001 12 ACCGTTC 60 0.0061063934 11.026314 8 GTCCTAT 255 0.0 11.025004 1 GGTATCA 1075 0.0 11.021341 1 GGACCTG 1720 0.0 11.016742 6 >>END_MODULE