FastQCFastQC Report
Thu 26 May 2016
SRR1512615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898966
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65760.34629371984543167No Hit
GTCCTACAGTGGACATTTCTAAATT57570.303164985576361No Hit
CTGTAGGACGTGGAATATGGCAAGA57390.30221710130671114No Hit
GTCCTAAAGTGTGTATTTCTCATTT56500.2975303401956644No Hit
CTTTAGGACGTGAAATATGGCGAGG51870.27314865037078073No Hit
GGTATCAACGCAGAGTACTTTTTTT45950.2419737899467394No Hit
TATCAACGCAGAGTACTTTTTTTTT45740.2408679249654812No Hit
GTCCTACAGTGTGCATTTCTCATTT34060.179360767912643No Hit
CTGAAGGACCTGGAATATGGCGAGA27380.14418372946119098No Hit
CTGTAGGACCTGGAATATGGCGAGA25600.13481020723909748No Hit
TTTCTAAATTTTCCACCTTTTTCAG23000.12111854556637665No Hit
ATTTAGAAATGTCCACTGTAGGACG22800.1200653408223212No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19570.1030560842058257No Hit
GAATATGGCAAGAAAACTGAAAATC19560.10300342396862291No Hit
GTCCTTCAGTGTGCATTTCTCATTT19370.10200287946177025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1950.014.12223418
TAGGACC10400.012.5180324
GGTATCA12900.011.9497331
AATGTCC9200.011.6678478
CCAACGA1406.85759E-1011.53053419
GGCGAGG13450.011.296041519
GGACGTA600.005876532811.0833186
AAATGTC9800.010.8568427
TGTCCAC10500.010.85655710
CCAACGT1053.485633E-610.85226819
AGGACGT30800.010.7374285
TGTAGGA34800.010.7315442
ACCTTTT8600.010.71143315
CACCTTT8600.010.601006514
GGACGTG30100.010.5415136
GACGTGG15150.010.5343617
AGGACCT20650.010.4926825
GGACCTG19250.010.4623236
CTGTAGG34750.010.4602351
TAGGACA8550.010.4474584
Produced by FastQC (version 0.11.5)