FastQCFastQC Report
Thu 26 May 2016
SRR1512614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205063
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58490.2652531923124192No Hit
GTCCTAAAGTGTGTATTTCTCATTT51160.2320115116892352No Hit
GTATCAACGCAGAGTACTTTTTTTT50320.22820209672013905No Hit
CTGTAGGACGTGGAATATGGCAAGA49850.2260706383445734No Hit
CTTTAGGACGTGAAATATGGCGAGG46380.2103341265079501No Hit
GTCCTACAGTGTGCATTTCTCATTT31650.14353331401415742No Hit
TATCAACGCAGAGTACTTTTTTTTT30460.13813664280793791No Hit
GGTATCAACGCAGAGTACTTTTTTT30340.1375924406694956No Hit
CTGAAGGACCTGGAATATGGCGAGA24320.1102916333909734No Hit
CTGTAGGACCTGGAATATGGCGAGA23300.10566591521421383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG400.00537246814.212764
AGTACGT500.001529754813.2652435
TAGGACC10900.012.3438054
CCAACGA1353.947207E-1011.92055919
GGTATCA13350.011.1900851
TACACCG600.00599253211.054375
GGCGAGG14300.010.98888219
TCCAACG2250.010.9376218
GACGTGG15850.010.820977
TGTAGGA34000.010.6739912
GTCCTAA16000.010.6526421
CTGTAGG31650.010.6437331
GGACCTG18800.010.5842126
AGGACGT31450.010.5447095
GGACGTG31350.010.3670136
GTCCTAC31750.010.3260081
ACGTGGA16750.010.3256178
AGGACCT19850.010.3105165
GTAGGAC34600.010.2693363
AATCCCG3350.010.17276819
Produced by FastQC (version 0.11.5)