FastQCFastQC Report
Thu 26 May 2016
SRR1512614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205063
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61590.279311747555512No Hit
GTCCTACAGTGGACATTTCTAAATT60850.27595583436845117No Hit
CTGTAGGACGTGGAATATGGCAAGA56730.25727156094859877No Hit
GTCCTAAAGTGTGTATTTCTCATTT56490.25618315667171415No Hit
CTTTAGGACGTGAAATATGGCGAGG52990.24031059430048032No Hit
TATCAACGCAGAGTACTTTTTTTTT40730.18471127582295835No Hit
GGTATCAACGCAGAGTACTTTTTTT40280.18267051780379973No Hit
GTCCTACAGTGTGCATTTCTCATTT33190.1505172414575003No Hit
CTGAAGGACCTGGAATATGGCGAGA29280.13278532177992194No Hit
CTGTAGGACCTGGAATATGGCGAGA27750.1258467445147826No Hit
ATTTAGAAATGTCCACTGTAGGACG24370.11051838428199105No Hit
TTTCTAAATTTTCCACCTTTTTCAG23120.10484961200655038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACG250.006022136719.0058542
CCAACGA1650.013.813663519
TCCAACG2900.013.09916318
TAGGACC11700.012.5893494
GGTATCA15250.012.2258031
CAACGAA550.003076673512.08695617
GGCGAGG13950.011.36904119
ACCTTTT8400.011.30608215
ACTGATC1850.011.2964218
CGAAATC1604.5474735E-1111.27858413
AGACCCG600.0058635211.0867485
CGGTTTC2350.010.91231413
AATACGG700.001495624310.85383814
GGCGAAA700.001495907210.85359319
GGACCTG20100.010.8247536
AGGACCT21100.010.7639795
ACGAAAT1605.456968E-1010.68545812
CACCTTT9500.010.49680414
AATGTCC9400.010.4087688
GATATAC5500.010.377191
Produced by FastQC (version 0.11.5)