FastQCFastQC Report
Thu 26 May 2016
SRR1512613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847961
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49410.26737577254065426No Hit
GTCCTAAAGTGTGTATTTCTCATTT46550.2518992554496551No Hit
GTATCAACGCAGAGTACTTTTTTTT46070.24930179803578106No Hit
CTGTAGGACGTGGAATATGGCAAGA44600.24134708470579194No Hit
CTTTAGGACGTGAAATATGGCGAGG42170.22819745654805487No Hit
GGTATCAACGCAGAGTACTTTTTTT30270.16380215816242874No Hit
TATCAACGCAGAGTACTTTTTTTTT29920.16190817879814565No Hit
GTCCTACAGTGTGCATTTCTCATTT24330.13165862266573808No Hit
CTGAAGGACCTGGAATATGGCGAGA23120.12511086543493072No Hit
CTGTAGGACCTGGAATATGGCGAGA19060.10314070480924652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG400.005374398514.2118165
TAGGACC9500.013.7630234
CTGTTCG500.00155466713.2366439
CCAACGA1201.05501385E-811.83352619
CCACCTT6900.011.53454213
GTATAGT1757.2759576E-1211.4723371
GTCTAGG1403.5233825E-911.4723361
TCCAACG1604.9112714E-1111.24032218
CACCTTT7300.011.03620514
GGTATCA12250.010.9806641
GTCCTAG2950.010.8889971
AGGACCT18750.010.8641445
GGCGAGG12750.010.76618819
GTAGAAC6450.010.7231283
GGACCTG18400.010.7103546
GTCCTAA15100.010.7030821
GTCCTAC24400.010.490841
TGTAGAA7200.010.3959642
TTAGGAC17750.010.3018983
TGGCGAG27600.010.28862518
Produced by FastQC (version 0.11.5)