FastQCFastQC Report
Thu 26 May 2016
SRR1512613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847961
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59800.3235999028118018No Hit
GTCCTACAGTGGACATTTCTAAATT54150.29302566450265993No Hit
CTGTAGGACGTGGAATATGGCAAGA51820.28041717330614657No Hit
GTCCTAAAGTGTGTATTTCTCATTT51450.2784149665496187No Hit
CTTTAGGACGTGAAATATGGCGAGG48920.2647242014306579No Hit
TATCAACGCAGAGTACTTTTTTTTT41830.2263575908798941No Hit
GGTATCAACGCAGAGTACTTTTTTT41410.22408481564275437No Hit
GTCCTACAGTGTGCATTTCTCATTT26950.14583641104980027No Hit
CTGAAGGACCTGGAATATGGCGAGA26220.14188611123286693No Hit
CTGTAGGACCTGGAATATGGCGAGA22000.11905013146922473No Hit
TTTCTAAATTTTCCACCTTTTTCAG21920.11861722190024573No Hit
ATTTAGAAATGTCCACTGTAGGACG19900.107686255283526No Hit
GAATATGGCAAGAAAACTGAAAATC18790.1016796350139424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTC500.001499492613.3002847
CCAACGA957.432209E-812.99429819
TAGGACC8200.012.6313964
CACCTTT7650.011.66966114
CCACCTT7850.011.61619713
TCCAACG1701.2732926E-1111.1718718
ACCTTTT8100.011.13859615
GGCGAGG13850.010.96991519
ACCGTGC700.001491704110.857088
TATTGCG700.001496764710.85267416
GGACGTG29100.010.8393066
AGGACGT29600.010.7869355
TTCCACC8200.010.77432911
GTCCTAT2650.010.768941
TAGGACT2700.010.5583544
AGGACCT17050.010.5338685
GGTATCA11850.010.4357231
GGACCTG17050.010.4201416
TTAGGAC17650.010.3908563
GACGTGA15550.010.3860747
Produced by FastQC (version 0.11.5)