FastQCFastQC Report
Thu 26 May 2016
SRR1512612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC69320.37305561176384483No Hit
CCCATGTACTCTGCGTTGATACCAC53950.29033973246767786No Hit
GTCCTACAGTGGACATTTCTAAATT47850.2575116996956142No Hit
GTCCTAAAGTGTGTATTTCTCATTT45010.24222782870009602No Hit
CTGTAGGACGTGGAATATGGCAAGA42910.23092637479495937No Hit
CTTTAGGACGTGAAATATGGCGAGG39550.21284404854674063No Hit
GTATCAACGCAGAGTACTTTTTTTT35500.19104838744397706No Hit
GAGTACATGGGAAGCAGTGGTATCA29710.15988866453410025No Hit
GGTATCAACGCAGAGTACTTTTTTT21830.11748130416625406No Hit
GTCCTACAGTGTGCATTTCTCATTT21650.11651260811724237No Hit
TATCAACGCAGAGTACTTTTTTTTT21100.1135527035230399No Hit
CTGAAGGACCTGGAATATGGCGAGA20960.11279927326269745No Hit
GTATCAACGCAGAGTACATGGGAAG19860.10687946407429254No Hit
CATGTACTCTGCGTTGATACCACTG19700.10601840091961545No Hit
GCGTTGATACCACTGCTTCCCATGT18720.10074438909721833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.014.1138244
AGTACCG550.003131072312.0585285
ACACCGT907.688674E-611.5800156
GTCCTAA13400.011.23531
GTATAGG1705.0931703E-1111.2176761
GGCGAGG12700.011.10620519
GGACGTG27950.011.0169276
AGGACGT28350.010.9617455
TGTAGGA27500.010.8874232
TCCAACG2450.010.81636218
GACGTGA14750.010.7913617
GTGTAGG2800.010.7537861
TTAGGAC17100.010.6935063
CCCTACA2050.010.6302792
GTAGGAC27300.010.6198343
CTAGTAC1252.3246503E-710.6115053
ATACTGC1701.4551915E-1010.5892116
CTTATAC1909.094947E-1210.471883
TTTAGGA18650.010.4654782
AGGACCT16200.010.3518315
Produced by FastQC (version 0.11.5)