FastQCFastQC Report
Thu 26 May 2016
SRR1512612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81110.43650520297411216No Hit
CCCATGTACTCTGCGTTGATACCAC62710.33748294018624797No Hit
GTCCTACAGTGGACATTTCTAAATT50290.27064291280443964No Hit
CTGTAGGACGTGGAATATGGCAAGA50210.2702123812271011No Hit
GTCCTAAAGTGTGTATTTCTCATTT50000.2690822358365874No Hit
CTTTAGGACGTGAAATATGGCGAGG46700.25132280827137266No Hit
GTATCAACGCAGAGTACTTTTTTTT45550.24513391684713115No Hit
GAGTACATGGGAAGCAGTGGTATCA36160.19460027295702004No Hit
GGTATCAACGCAGAGTACTTTTTTT29200.15714402572856706No Hit
TATCAACGCAGAGTACTTTTTTTTT27720.14917919154780407No Hit
GTCCTACAGTGTGCATTTCTCATTT23670.12738353044504047No Hit
CTGAAGGACCTGGAATATGGCGAGA23560.1267915495262No Hit
GTATCAACGCAGAGTACATGGGAAG22570.12146372125663557No Hit
CATGTACTCTGCGTTGATACCACTG22040.11861144955676775No Hit
GCGTTGATACCACTGCTTCCCATGT21940.11807328508509456No Hit
TATCAACGCAGAGTACATGGGAAGC19930.10725617920446374No Hit
TTTCTAAATTTTCCACCTTTTTCAG19290.10381192658575544No Hit
ATTTAGAAATGTCCACTGTAGGACG18980.10214361672356859No Hit
CTGTAGGACCTGGAATATGGCGAGA18870.1015516358047281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1151.8189894E-1214.86404619
CTACGAC456.750506E-414.7814433
GCGCCAC602.5725707E-514.24777913
TAGGACC6450.012.9644554
TCCAACG2000.012.34541818
GTCTAGG1353.6379788E-1011.9788321
GGCGAGG13350.011.09700419
GTCGAGG951.3660876E-510.99591819
AGGACCT15150.010.6629975
TTGGACT1355.6672434E-810.5581744
GGACCTG14750.010.498936
TTAGGAC17250.010.4112783
GACGTGA16150.010.3532787
AGGACGT29200.010.3161925
TAGAAAT6000.010.2942194
CACCTTT7600.010.24702514
GGACGTG28850.010.1427176
ACCTTTT7600.010.12178815
TGTAGGA25100.010.0702262
CTACACT3700.010.0159974
Produced by FastQC (version 0.11.5)