FastQCFastQC Report
Thu 26 May 2016
SRR1512611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120907
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40530.191097488008668No Hit
GTCCTACAGTGGACATTTCTAAATT33840.15955437932922092No Hit
GTCCTAAAGTGTGTATTTCTCATTT31150.14687112636244776No Hit
GTACATGGGAAGCAGTGGTATCAAC28770.13564951221340682No Hit
CTGTAGGACGTGGAATATGGCAAGA26930.12697397858557682No Hit
CTTTAGGACGTGAAATATGGCGAGG25260.1190999888255355No Hit
GGTATCAACGCAGAGTACTTTTTTT25250.1190528391862538No Hit
TATCAACGCAGAGTACTTTTTTTTT24800.11693110541857799No Hit
CCCATGTACTCTGCGTTGATACCAC22320.1052379948767202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT400.00537612514.21126656
CCAACGA1301.4551915E-1113.10504119
TAGGACC5800.012.5768934
GTCTTAA2500.012.0574881
GCGACCA752.1701201E-411.3448029
GTATTAA2350.011.1168341
AAGCGAC600.005996036311.0534697
GTATTAG1852.0008883E-1110.8626021
GCCTAAG1404.2597094E-810.76561451
GGCGAGG8450.010.75285319
AGGACGT18350.010.73835855
GTGCGCC803.87726E-410.6556711
TGGCGAG18300.010.65153418
ATCCCGT1701.4733814E-1010.58451910
AAGTCGT1355.9195372E-810.5271137
GGACGTG17750.010.5150026
TTAGGAC12850.010.4690223
TCCAACG2350.010.46891118
GTGTAGG3650.010.4608351
TGTAGGA19750.010.3131722
Produced by FastQC (version 0.11.5)