FastQCFastQC Report
Thu 26 May 2016
SRR1512611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120907
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46810.22070746147756595No Hit
GTACATGGGAAGCAGTGGTATCAAC34180.16115746706479822No Hit
GTCCTACAGTGGACATTTCTAAATT34090.16073312031126305No Hit
TATCAACGCAGAGTACTTTTTTTTT32090.15130319245492613No Hit
CTTTAGGACGTGAAATATGGCGAGG31910.15045449894785579No Hit
CTGTAGGACGTGGAATATGGCAAGA31510.1485685133765884No Hit
GGTATCAACGCAGAGTACTTTTTTT30830.14536233790543385No Hit
GTCCTAAAGTGTGTATTTCTCATTT29680.13994012938804012No Hit
CCCATGTACTCTGCGTTGATACCAC24960.11768549964708495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.013.2371944
TCCAACG2000.012.34641518
GGACCGA550.003068707912.09110456
ATACCGT658.024449E-411.6924976
GACAGTC2900.011.4654327
ATTATAC2250.011.4023363
GTGTAAG2350.011.3340351
CCAACGA1201.2814962E-711.07985419
ATAGGAC3200.010.9866263
AGGACCT14400.010.8879045
GGACCTG13950.010.6238286
AGTCGTC1451.4451871E-810.4821868
CCGGTTT1451.4462785E-810.48169112
TGTAGGA21400.010.4787822
GTAGGAC21050.010.4724553
ACCGTGG1303.8456164E-710.2302118
GGCGAGG9100.010.22755719
CTGTAGG21100.010.1436541
GTGCTAA1803.8562575E-1010.0409651
CTAGACT2002.5465852E-119.9770434
Produced by FastQC (version 0.11.5)