FastQCFastQC Report
Thu 26 May 2016
SRR1512610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978908
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44280.22375977054011606No Hit
GTCCTAAAGTGTGTATTTCTCATTT43240.21850434684179357No Hit
CTGTAGGACGTGGAATATGGCAAGA41140.20789243360479617No Hit
GTATCAACGCAGAGTACTTTTTTTT39980.20203061486435953No Hit
CTTTAGGACGTGAAATATGGCGAGG36390.18388929652111166No Hit
GTACATGGGAAGCAGTGGTATCAAC28590.1444736187836928No Hit
GGTATCAACGCAGAGTACTTTTTTT25900.13088026325630095No Hit
TATCAACGCAGAGTACTTTTTTTTT25640.1295664073317203No Hit
CCCATGTACTCTGCGTTGATACCAC20420.10318822299975543No Hit
CTGAAGGACCTGGAATATGGCGAGA19940.10076264283129888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT250.006128583618.9481586
TAGGACC7750.013.5693254
TCCAACG1950.011.64353818
GTATTAA1900.011.6258811
GGCGAGG11550.011.38954819
GGACCTG14500.011.2382186
AGGACCT15250.011.1825195
CTAGGAC2800.011.166733
CCACCTT6800.010.87091713
TGTAGGA23900.010.822682
CCTACAC2650.010.7261913
GTGTAGG4050.010.6603191
GGACGTG24100.010.4568676
GACGTGG11400.010.3890357
CAAGTCG2100.010.3763716
AATGTCC7200.010.3680318
AGTCGTC2100.010.3492828
GTAGGAC24000.010.3433253
AGGACGT24550.010.3423765
CTGTAGG22100.010.1768291
Produced by FastQC (version 0.11.5)