FastQCFastQC Report
Thu 26 May 2016
SRR1512610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978908
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51740.26145732899154484No Hit
CTGTAGGACGTGGAATATGGCAAGA47580.2404356341982548No Hit
GTCCTACAGTGGACATTTCTAAATT47100.23801005402979825No Hit
GTCCTAAAGTGTGTATTTCTCATTT45680.230834379364781No Hit
CTTTAGGACGTGAAATATGGCGAGG42490.2147143778285802No Hit
GGTATCAACGCAGAGTACTTTTTTT34690.17529870009116139No Hit
TATCAACGCAGAGTACTTTTTTTTT34430.17398484416658078No Hit
GTACATGGGAAGCAGTGGTATCAAC32950.16650597198050643No Hit
CCCATGTACTCTGCGTTGATACCAC23710.11981355373771797No Hit
CTGAAGGACCTGGAATATGGCGAGA23640.11945982329648473No Hit
GTCCTACAGTGTGCATTTCTCATTT21910.11071762810600594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACA400.00529069114.24556517
CCAACGA1203.6379788E-1214.24520519
TAGGACC8100.013.7238484
TCCAACG2050.013.43484218
CCCGTTT1551.8189894E-1212.25703712
GGACCGT658.0205547E-411.6931016
AACCGGC658.039284E-411.6898515
ATCCCGT1657.2759576E-1211.51447810
CCAACGT752.0808539E-411.39616519
GACGTGG12850.011.1638997
AGGACGT24750.011.05697255
GGACGTG24600.011.04546456
TGTCCAC7450.010.965810
CTTGCAC1303.26163E-810.9628363
AATGTCC7150.010.8944678
GAAATGT7550.010.696096
AAATGTC7300.010.6716877
GAACAGT4900.010.6639896
GGCGAGG11250.010.6364219
CACCTTT8500.010.61568414
Produced by FastQC (version 0.11.5)