FastQCFastQC Report
Thu 26 May 2016
SRR1512609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54950.2910109652084343No Hit
GTCCTAAAGTGTGTATTTCTCATTT52050.2756527887006188No Hit
CTGTAGGACGTGGAATATGGCAAGA50850.26929768118014347No Hit
CTTTAGGACGTGAAATATGGCGAGG44940.2379987766418023No Hit
GTATCAACGCAGAGTACTTTTTTTT41900.22189917092326475No Hit
GTACATGGGAAGCAGTGGTATCAAC32170.170369840778077No Hit
GTCCTACAGTGTGCATTTCTCATTT30260.16025462797465373No Hit
GGTATCAACGCAGAGTACTTTTTTT25830.13679368937823216No Hit
TATCAACGCAGAGTACTTTTTTTTT25310.13403980945269284No Hit
CTGAAGGACCTGGAATATGGCGAGA24650.1305445003164314No Hit
CCCATGTACTCTGCGTTGATACCAC24400.12922051958299902No Hit
ATTTAGAAATGTCCACTGTAGGACG20520.10867233860012869No Hit
CTGTAGGACCTGGAATATGGCGAGA20380.10793090938940655No Hit
GTCCTTCAGTGTGCATTTCTCATTT19580.10369417104242298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1350.014.72543119
TCCAACG1700.013.91813818
TAGGACC9650.013.8427064
AGGACCT19650.012.0533195
AAACGGG658.267227E-411.6508919
GGCGAGG13500.011.56998119
GGACCTG19550.011.4849946
GCAATAC1001.9937233E-611.3692963
CGGTAGG802.2648153E-411.2925081
TTGGACC1451.2751116E-911.1073424
ACGTGTA600.006105716311.0264748
GTCCTAG2750.010.9503111
GACGTGA16600.010.9007027
GCCTTAC1207.5052594E-710.8742681
GGACGTG30950.010.8666816
AGGACGT31700.010.8486725
GTCGAGG1053.6174042E-610.81868419
GTCCTAT2600.010.8099211
GTCCTAC28850.010.7510411
TGTAGGA33300.010.6978372
Produced by FastQC (version 0.11.5)