FastQCFastQC Report
Thu 26 May 2016
SRR1512609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58440.30949373624715015No Hit
CTGTAGGACGTGGAATATGGCAAGA57490.3044626094601071No Hit
GTCCTAAAGTGTGTATTTCTCATTT55190.29228198671252936No Hit
CTTTAGGACGTGAAATATGGCGAGG52800.27962473090091594No Hit
GTATCAACGCAGAGTACTTTTTTTT51310.27173380572965905No Hit
GTACATGGGAAGCAGTGGTATCAAC38760.20526997291135418No Hit
TATCAACGCAGAGTACTTTTTTTTT34480.18260342275499208No Hit
GGTATCAACGCAGAGTACTTTTTTT33560.17773117365596094No Hit
GTCCTACAGTGTGCATTTCTCATTT31850.16867514543928355No Hit
CTGAAGGACCTGGAATATGGCGAGA27860.147544412933703No Hit
CCCATGTACTCTGCGTTGATACCAC27390.14505532915485014No Hit
CTGTAGGACCTGGAATATGGCGAGA24390.12916756035366173No Hit
ATTTAGAAATGTCCACTGTAGGACG22740.1204292875130081No Hit
TTTCTAAATTTTCCACCTTTTTCAG22340.11831091833951633No Hit
GTCCTTCAGTGTGCATTTCTCATTT21050.11147917775500532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1600.014.24439818
CCAACGA1006.6393113E-1014.24402319
TAGGACC9300.013.79330354
ATCCCGT1051.371518E-913.57148610
CCCGTTT1201.0028089E-811.87410512
GGACCGG658.019193E-411.6932856
TCCCGTT1157.075505E-811.56526611
TAGAAAT7750.011.5250714
GTCCTAG3200.011.2950611
GGACCTG18400.011.2047426
AATCCCG2800.011.19173119
AGGACCG855.309452E-511.179485
GGCGAGG14050.011.15190419
ACCTTTT7750.011.15163515
CGTTTCC1201.280223E-711.08044314
TTTAGAA9200.011.0513022
AGGACCT18800.011.0189225
ATAGGAC2950.010.9515193
ACTGTTC5950.010.8571878
CCTTCGC700.001495237610.85402515
Produced by FastQC (version 0.11.5)