FastQCFastQC Report
Thu 26 May 2016
SRR1512608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120856
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49970.2356124131011252No Hit
GTCCTAAAGTGTGTATTTCTCATTT45870.21628059613665426No Hit
CTGTAGGACGTGGAATATGGCAAGA44730.21090540800506966No Hit
GTATCAACGCAGAGTACTTTTTTTT44260.2086893216701181No Hit
CTTTAGGACGTGAAATATGGCGAGG41220.19435548665255917No Hit
GTCCTACAGTGTGCATTTCTCATTT29560.13937768523652716No Hit
TATCAACGCAGAGTACTTTTTTTTT27470.12952317366195534No Hit
GGTATCAACGCAGAGTACTTTTTTT27340.1289102136118624No Hit
GTACATGGGAAGCAGTGGTATCAAC21500.10137416213076231No Hit
CTGTAGGACCTGGAATATGGCGAGA21400.10090265439992153No Hit
CTGAAGGACCTGGAATATGGCGAGA21360.10071405130758523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9000.012.5285894
ACCTTTT7500.011.62784815
GTCTTAG2850.011.6182481
GGCGAGG13200.011.47228319
CACCTTT7950.011.20813814
GCCCTAT905.460374E-511.1488251
ACACCGT951.4000119E-510.971786
TAGGACT3250.010.7873824
GTGTAGG3200.010.6610631
AGGACCT18150.010.5978345
CCACCTT8250.010.567512513
TGTAGGA28900.010.557372
GGACCTG17350.010.5406126
CTAGGAC3600.010.5282263
GACGTGG14150.010.5135957
TGGCGAG28850.010.46324518
GGACGTG28150.010.4349736
AGGACGT28900.010.3606495
CTAGGGT1106.2373183E-610.3368044
GTACTAA1759.986252E-1010.3206251
Produced by FastQC (version 0.11.5)