FastQCFastQC Report
Thu 26 May 2016
SRR1512608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120856
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57310.2702210805448366No Hit
GTCCTACAGTGGACATTTCTAAATT56500.2664018679250265No Hit
CTGTAGGACGTGGAATATGGCAAGA51650.2435337429792499No Hit
GTCCTAAAGTGTGTATTTCTCATTT50350.23740414247832006No Hit
CTTTAGGACGTGAAATATGGCGAGG46900.22113712576431405No Hit
TATCAACGCAGAGTACTTTTTTTTT38830.1830864518854651No Hit
GGTATCAACGCAGAGTACTTTTTTT37880.17860712844247795No Hit
GTCCTACAGTGTGCATTTCTCATTT31130.146780356610727No Hit
CTGAAGGACCTGGAATATGGCGAGA25420.11985726517971988No Hit
GTACATGGGAAGCAGTGGTATCAAC24620.11608520333299384No Hit
CTGTAGGACCTGGAATATGGCGAGA24270.11443492627505121No Hit
ATTTAGAAATGTCCACTGTAGGACG21810.10283583609636864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9700.013.1278674
ACCTGCG801.9996296E-613.0629377
ACCGTCC802.8699937E-511.8739988
ACCGTGC658.031221E-411.691328
ACCGACC600.005880548611.0823978
AGGACGT28450.011.0228215
GGACGTG28100.010.8869996
TGTCGAG1408.403731E-910.85315518
GACGTGG14100.010.8478817
ATAGGAC3100.010.728953
TGTAGGA29750.010.6686722
CACCTTT7500.010.63734614
AGGACCT21400.010.61317355
ACCTTTT7600.010.49713315
GTAGGAC29900.010.42445853
AGGACTG4200.010.4080575
CTCGAAC1659.349606E-1010.3598318
CTGTAGG29750.010.32778551
GTGCGCC1201.5263231E-610.29055511
GGACCTG20900.010.2280976
Produced by FastQC (version 0.11.5)