FastQCFastQC Report
Thu 26 May 2016
SRR1512607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890779
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66430.35133667128733714No Hit
GTCCTAAAGTGTGTATTTCTCATTT64010.3385377138205999No Hit
CTGTAGGACGTGGAATATGGCAAGA58650.3101896096793967No Hit
CTTTAGGACGTGAAATATGGCGAGG54300.2871832191916665No Hit
GTATCAACGCAGAGTACTTTTTTTT42630.22546262677975584No Hit
GTCCTACAGTGTGCATTTCTCATTT32670.17278592580095292No Hit
CTGAAGGACCTGGAATATGGCGAGA27820.14713512261348363No Hit
GGTATCAACGCAGAGTACTTTTTTT27220.14396182737379673No Hit
TATCAACGCAGAGTACTTTTTTTTT26120.13814411943437072No Hit
GTACATGGGAAGCAGTGGTATCAAC26030.13766812514841767No Hit
ATTTAGAAATGTCCACTGTAGGACG24120.1275664686354143No Hit
GTCCTTCAGTGTGCATTTCTCATTT23470.12412873212575347No Hit
CTGTAGGACCTGGAATATGGCGAGA22330.11809947117034832No Hit
TTTCTAAATTTTCCACCTTTTTCAG21990.11630127053452571No Hit
GAATATGGCAAGAAAACTGAAAATC19750.10445430163969455No Hit
CCCATGTACTCTGCGTTGATACCAC19120.10112234163802328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG350.002209103216.2427047
TAACGCA350.002209103216.2427044
TAGGACC10500.013.26487354
TCCAACG1750.011.89549918
GTCTTAT1508.58563E-1011.3746421
CCAACGA1355.0240487E-911.21580419
CGCCACC855.4383127E-511.15318214
GGCGAGG17250.011.08170119
GCGCCAC951.3984887E-510.972713
GTCCTAA19000.010.8816251
TTAGGAC22700.010.8108683
GACGTGA19600.010.7801287
TCTTATA3000.010.7385172
GGACGTG35800.010.71882256
AGGACCT21050.010.7127265
AGGACGT37000.010.6528725
TGTAGGA36800.010.6338092
AATGTCC9350.010.6128988
CTGTAGG33900.010.5693591
GTCCTAG2900.010.3825341
Produced by FastQC (version 0.11.5)