FastQCFastQC Report
Thu 26 May 2016
SRR1512607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890779
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA69630.3682609125656674No Hit
GTCCTACAGTGGACATTTCTAAATT68110.3602218979584605No Hit
GTCCTAAAGTGTGTATTTCTCATTT66180.3500144649374676No Hit
CTTTAGGACGTGAAATATGGCGAGG64580.34155234429830245No Hit
GTATCAACGCAGAGTACTTTTTTTT52470.27750466871062135No Hit
GGTATCAACGCAGAGTACTTTTTTT35010.18516177723573193No Hit
TATCAACGCAGAGTACTTTTTTTTT34910.18463289469578412No Hit
CTGAAGGACCTGGAATATGGCGAGA33620.17781030993045724No Hit
GTCCTACAGTGTGCATTTCTCATTT33570.17754586866048333No Hit
GTACATGGGAAGCAGTGGTATCAAC30610.16189094547802782No Hit
ATTTAGAAATGTCCACTGTAGGACG27690.14644757531155148No Hit
CTGTAGGACCTGGAATATGGCGAGA27510.1454955867396454No Hit
TTTCTAAATTTTCCACCTTTTTCAG25340.1340188356227777No Hit
GTCCTTCAGTGTGCATTTCTCATTT24970.13206197022497076No Hit
GAATATGGCAAGAAAACTGAAAATC23060.1219603137119674No Hit
CCCATGTACTCTGCGTTGATACCAC20710.10953157402319362No Hit
GGAATATGGCGAGAAAACTGAAAAT19940.10545917846559541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11750.012.7785794
GGCGAGG15050.012.42967719
TATGCGC550.00307059612.0910
CCCGTTT1052.724464E-711.76070912
TTATACT2400.011.4828614
CCAACGA855.3482545E-511.17149219
GCGTGCA600.005879746711.08249959
AGGACCT24250.010.8556135
TCCAACG1053.4843524E-610.85259318
CGTTTCC1158.8601337E-710.73519914
GATCTAT803.7209253E-410.703121
ATTTAGA9600.010.7031191
GTCCTAA17250.010.6996731
GGACCTG23450.010.6152416
TTTAGAA10700.010.5690992
TGTCCAC10250.010.472383510
TAGAAAT9850.010.4195954
TGTCGAG1659.367795E-1010.35929518
GTCGAGG1106.078517E-610.3590219
TGGCGAG38650.010.34365918
Produced by FastQC (version 0.11.5)