FastQCFastQC Report
Thu 26 May 2016
SRR1512606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185521
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52290.23925645189407924No Hit
GTACATGGGAAGCAGTGGTATCAAC46140.2111167085559919No Hit
GTCCTAAAGTGTGTATTTCTCATTT43800.20040987938345134No Hit
CTGTAGGACGTGGAATATGGCAAGA43640.19967778849985882No Hit
CTTTAGGACGTGAAATATGGCGAGG38110.1743748973356925No Hit
GTATCAACGCAGAGTACTTTTTTTT37280.17057717587705631No Hit
CCCATGTACTCTGCGTTGATACCAC35050.16037365918698562No Hit
GTCCTACAGTGTGCATTTCTCATTT25710.11763785385727246No Hit
TATCAACGCAGAGTACTTTTTTTTT22450.10272150210407496No Hit
GGTATCAACGCAGAGTACTTTTTTT22440.10267574642385043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA551.9953292E-413.78970116
TAGGACC8100.012.164554
GTATAGG2450.011.4684241
ATTTAGA7100.011.4482331
TCCAACG2100.011.26546618
TATTGCG600.005967868511.0604916
GTCCTAC25000.010.8376611
GGCGAGG13650.010.81683619
GTACTAG1404.3421096E-810.7516471
TTAGGAC17400.010.5635573
GTCCTAA14850.010.5416831
GACGTGA15300.010.5272567
TAGAAAT8300.010.5016484
TATACTG3650.010.3828085
TTTAGAA8550.010.305632
GGACGTG28650.010.2516746
AGGACGA1303.988407E-710.2031075
AACAGTC2053.6379788E-1210.16778857
AATGTCC8000.010.1612748
ACAGTCC2800.010.1275168
Produced by FastQC (version 0.11.5)