FastQCFastQC Report
Thu 26 May 2016
SRR1512606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185521
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55460.253761002525256No Hit
GTACATGGGAAGCAGTGGTATCAAC53360.2441523096781042No Hit
CTGTAGGACGTGGAATATGGCAAGA51980.23783802580711874No Hit
GTCCTAAAGTGTGTATTTCTCATTT47640.2179800605896718No Hit
CTTTAGGACGTGAAATATGGCGAGG44810.20503120308612913No Hit
GTATCAACGCAGAGTACTTTTTTTT44810.20503120308612913No Hit
CCCATGTACTCTGCGTTGATACCAC39510.18078069256712703No Hit
TATCAACGCAGAGTACTTTTTTTTT30180.1380906429176384No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.13529954642394193No Hit
GTCCTACAGTGTGCATTTCTCATTT28450.13017491023879432No Hit
CTGAAGGACCTGGAATATGGCGAGA26360.12061197307186708No Hit
GAGTACATGGGAAGCAGTGGTATCA23150.10592439971979221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG551.9566415E-413.8215794
TAGGACC8950.012.4220474
CCAACGA1551.8189894E-1212.25266119
GACCGTC550.0030666412.092227
ACCCGTC550.0030666412.092227
TCCAACG2250.011.81701218
TCGAAAA658.0477144E-411.68849214
GGCGAGG11600.011.54232419
GTATAGG2350.011.3341271
AGGACCT19150.011.2147585
AAATGTC6500.011.1088977
AATGTCC7000.010.8575748
GGACCTG19400.010.6771496
GGACGTG26650.010.6247716
TAGGACT3950.010.584884
TGTAGGA28850.010.5725472
GAATACG909.5320545E-510.55404313
TGGCGAG28550.010.54351218
GTAGGAC28100.010.4827643
AGGACGT27300.010.4774135
Produced by FastQC (version 0.11.5)