FastQCFastQC Report
Thu 26 May 2016
SRR1512605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389487
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69020.288848610601355No Hit
GTCCTAAAGTGTGTATTTCTCATTT64360.2693465166372531No Hit
CTGTAGGACGTGGAATATGGCAAGA58960.2467475236316414No Hit
CTTTAGGACGTGAAATATGGCGAGG51800.21678293290568226No Hit
GTATCAACGCAGAGTACTTTTTTTT40200.16823694793066463No Hit
GTCCTACAGTGTGCATTTCTCATTT37810.15823480102632909No Hit
GTACATGGGAAGCAGTGGTATCAAC30120.12605216098685618No Hit
CTGAAGGACCTGGAATATGGCGAGA28900.12094646256706984No Hit
CTGTAGGACCTGGAATATGGCGAGA25450.10650821703570683No Hit
GTCCTTCAGTGTGCATTTCTCATTT24580.1028672681625805No Hit
TATCAACGCAGAGTACTTTTTTTTT24410.10215581838277422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1550.014.04609619
TCCAACG2800.013.51837418
CCAACGT1251.09139364E-1012.87355119
TAGGACC12100.012.3718094
GGACCGT701.118748E-412.1819036
AGGACCG951.074699E-611.9679335
GATATAC7500.011.5111921
GTGTAGG4200.011.2339291
GTCCTAA18150.011.0620721
CCATGCG600.00609853811.0284129
TGTAGGA39150.010.7211812
AGGACGT38800.010.6955695
GGACGTA803.8659223E-410.6591646
GTCCACG853.967058E-410.629351
GGCGAGG17200.010.6215619
CTGTAGG36350.010.6049811
TTAGGAC21650.010.5470213
GGACGTG37750.010.5415146
GAAGCGA909.778641E-510.527576
GTAGGAC39900.010.5196543
Produced by FastQC (version 0.11.5)