FastQCFastQC Report
Thu 26 May 2016
SRR1512605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389487
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74320.3110291037364924No Hit
GTCCTAAAGTGTGTATTTCTCATTT69920.29261510943562363No Hit
CTGTAGGACGTGGAATATGGCAAGA68710.2875512610028847No Hit
CTTTAGGACGTGAAATATGGCGAGG61640.25796332016035245No Hit
GTATCAACGCAGAGTACTTTTTTTT53620.22439963054831433No Hit
GTCCTACAGTGTGCATTTCTCATTT40370.1689483977104709No Hit
GTACATGGGAAGCAGTGGTATCAAC35440.14831635409608843No Hit
CTGAAGGACCTGGAATATGGCGAGA35210.14735380439399756No Hit
GGTATCAACGCAGAGTACTTTTTTT34090.1426666058446855No Hit
TATCAACGCAGAGTACTTTTTTTTT33290.13931860688089118No Hit
CTGTAGGACCTGGAATATGGCGAGA30180.12630326090914074No Hit
ATTTAGAAATGTCCACTGTAGGACG26990.11295311504101088No Hit
GTCCTTCAGTGTGCATTTCTCATTT26140.10939586614197942No Hit
TTTCTAAATTTTCCACCTTTTTCAG25620.10721966681551312No Hit
CCCATGTACTCTGCGTTGATACCAC24960.1044575676703828No Hit
GAATATGGCAAGAAAACTGAAAATC24370.1019884184345845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG400.00526905614.25453954
TAGGACC11150.013.0400274
AAGGCGT1102.748493E-912.9546036
TACACCG1001.4373109E-712.3541935
GGCGAGG15850.012.28146419
GGACCGT802.8677414E-511.8750526
GATATAC8450.011.2615151
CCAACGA1950.011.20001919
GGCGTGT855.329705E-511.1755848
AAAGGCG1201.270273E-711.0870965
AGCCGCT600.005877031511.0833827
TCCAACG2400.011.07874318
TGTAGGA37650.010.8784022
AGGACCT23550.010.7743575
TTAGGAC24200.010.7593993
GGACGTG36650.010.62760456
AATGTCC8900.010.5665778
ACTGTTC9350.010.5660078
GGACCTG22500.010.4711586
AGGACGT38150.010.4622675
Produced by FastQC (version 0.11.5)