FastQCFastQC Report
Thu 26 May 2016
SRR1512604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265579
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT75200.33192398058068157No Hit
GTCCTAAAGTGTGTATTTCTCATTT66370.2929493961587744No Hit
CTGTAGGACGTGGAATATGGCAAGA66030.2914486760338086No Hit
CTTTAGGACGTGAAATATGGCGAGG56430.24907540191712582No Hit
GTATCAACGCAGAGTACTTTTTTTT41830.18463271419800412No Hit
GTCCTACAGTGTGCATTTCTCATTT39290.17342145208796514No Hit
CTGAAGGACCTGGAATATGGCGAGA30500.1346234229748775No Hit
CTGTAGGACCTGGAATATGGCGAGA28260.12473632568098486No Hit
GTCCTTCAGTGTGCATTTCTCATTT25790.11383403536138002No Hit
GGTATCAACGCAGAGTACTTTTTTT25450.11233331523641417No Hit
GTACATGGGAAGCAGTGGTATCAAC25180.11114156690188248No Hit
TATCAACGCAGAGTACTTTTTTTTT24320.10734562776226297No Hit
ATTTAGAAATGTCCACTGTAGGACG24250.10703665597182883No Hit
TTTCTAAATTTTCCACCTTTTTCAG22730.10032755423668739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG552.006416E-413.7807365
GCACTCG707.458808E-613.53705713
GTCTTAA2400.012.9622461
GTCGAGG1102.901288E-912.9076919
TAGGACC11200.012.8579214
TGGACCG658.2106394E-411.6606245
CGTGCGC752.1345717E-411.3650710
CACCTTT9750.011.18345414
GTCCTAA18250.011.1625391
GTCCTAC38750.011.1100351
AGCCGTC600.00599594811.0535447
TGTAGGA40750.010.9276062
GTCCTAT3950.010.9244911
TCTATAC2950.010.9194823
TCCAACG2100.010.81523518
GCCTTAA1301.8076025E-710.8072331
GGCGAGG16650.010.6879319
TAGAAAT10200.010.681764
GTTCTAG2550.010.6255991
GTGTAGG4250.010.6255991
Produced by FastQC (version 0.11.5)