FastQCFastQC Report
Thu 26 May 2016
SRR1512604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265579
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80040.35328717294784245No Hit
CTGTAGGACGTGGAATATGGCAAGA75180.33183570292627185No Hit
GTCCTAAAGTGTGTATTTCTCATTT70820.3125911742649451No Hit
CTTTAGGACGTGAAATATGGCGAGG67860.2995260814123012No Hit
GTATCAACGCAGAGTACTTTTTTTT54010.23839380573354535No Hit
GTCCTACAGTGTGCATTTCTCATTT43160.1905031782162529No Hit
CTGAAGGACCTGGAATATGGCGAGA35820.15810527904787253No Hit
GGTATCAACGCAGAGTACTTTTTTT34950.15426520108104816No Hit
TATCAACGCAGAGTACTTTTTTTTT34460.15210239854800914No Hit
CTGTAGGACCTGGAATATGGCGAGA32370.14287738366218966No Hit
GTACATGGGAAGCAGTGGTATCAAC28840.12729637765886778No Hit
ATTTAGAAATGTCCACTGTAGGACG28170.12433907623614095No Hit
TTTCTAAATTTTCCACCTTTTTCAG28020.1236769938280678No Hit
GTCCTTCAGTGTGCATTTCTCATTT27490.12133763598620925No Hit
GAATATGGCAAGAAAACTGAAAATC25610.11303953647169222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA400.00527085514.253762
TAGGACC13100.014.1449544
GTCGTAC551.9381402E-413.8371091
TGGCGAA1153.8016879E-1013.21299918
GTCGAGG1155.3623808E-912.38609319
AGGACCT25000.011.6693355
AGGACCG1001.9240415E-611.403265
CCAACGT1251.8446372E-811.395205519
GGCGAGG17100.011.328567519
GGACCTG25150.011.2947216
TGTAGGA41500.011.288522
CCACCTT9150.011.21087513
CCGGTTT1950.011.20387812
CCAACGA1451.2259989E-911.13324719
CTGTAGG40600.011.0594631
ACCTTTT9750.011.00732715
GTAGGAC42900.010.8093783
AGGACGT40550.010.7330315
TCCAACG2850.010.66312318
GCCGGTT2050.010.65781811
Produced by FastQC (version 0.11.5)