FastQCFastQC Report
Thu 26 May 2016
SRR1512603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433100
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72050.2961242858904279No Hit
CTGTAGGACGTGGAATATGGCAAGA62890.258476840244955No Hit
GTCCTAAAGTGTGTATTTCTCATTT59080.24281780444700174No Hit
GTATCAACGCAGAGTACTTTTTTTT53180.21856890386749414No Hit
CTTTAGGACGTGAAATATGGCGAGG51310.21088323537873493No Hit
GTCCTACAGTGTGCATTTCTCATTT38140.15675475730549507No Hit
TATCAACGCAGAGTACTTTTTTTTT32430.13328675352431055No Hit
GGTATCAACGCAGAGTACTTTTTTT30940.1271628786321976No Hit
CTGTAGGACCTGGAATATGGCGAGA28460.11697012042250626No Hit
CTGAAGGACCTGGAATATGGCGAGA28020.11516172783691586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12250.012.9941764
TCCAACG3600.012.8815118
GGCGAGG16200.012.26962519
TGGACCG550.003130658312.0589895
CCAACGT1650.012.0465419
CCAACGA2400.011.83142419
TAAGACG658.206205E-411.661444
GTCCTAG3750.011.2415391
GTCCTAA19050.011.222571
AAATGTC7800.011.0543027
AGGACGT36100.010.9972065
GGACGTG35850.010.91554456
AGGACCT23300.010.89819055
TTAGGAC22550.010.8407093
TGTAGGA38200.010.7897252
CGGTTTC2550.010.77651313
AATGTCC8350.010.7522948
GACGTGA19850.010.6923237
GTAGGAC38250.010.6517643
GACGTGG17250.010.6011457
Produced by FastQC (version 0.11.5)