FastQCFastQC Report
Thu 26 May 2016
SRR1512603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433100
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76760.3154823065225432No Hit
CTGTAGGACGTGGAATATGGCAAGA74470.30607044511117504No Hit
GTATCAACGCAGAGTACTTTTTTTT68090.27984875262011427No Hit
GTCCTAAAGTGTGTATTTCTCATTT62340.256216349512967No Hit
CTTTAGGACGTGAAATATGGCGAGG60140.247174386585015No Hit
TATCAACGCAGAGTACTTTTTTTTT44310.1821133533352513No Hit
GGTATCAACGCAGAGTACTTTTTTT43710.17964736344580987No Hit
GTCCTACAGTGTGCATTTCTCATTT39860.16382392832189388No Hit
CTGAAGGACCTGGAATATGGCGAGA34470.1416711191484115No Hit
CTGTAGGACCTGGAATATGGCGAGA31030.1275327771156138No Hit
ATTTAGAAATGTCCACTGTAGGACG24760.10176318277095063No Hit
GTCCTTCAGTGTGCATTTCTCATTT24380.10020138917430439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13800.013.772024
TAAGACG701.0885044E-412.217754
AGTCGTC2200.012.09110458
CGTGCGC802.8686509E-511.87470510
CGAGACG907.4981854E-611.60652718
AGGACCT26100.011.432365
AAGTCGT2250.011.400427
TGTAGGA40650.011.244042
TCCAACG3050.011.20868918
CCAACGT1701.2732926E-1111.17182819
GGCGAGG16700.011.14506919
CACCGTT600.00587566611.0837427
CAAGTCG2150.011.0473726
GGACCTG26350.010.9249516
GTAGGAC40800.010.8300543
CTGTAGG41800.010.8100761
TAGGACT4500.010.7697214
GGTATCA18450.010.7245321
CCACCTT8800.010.68591613
CACCTTT8650.010.64984914
Produced by FastQC (version 0.11.5)