FastQCFastQC Report
Thu 26 May 2016
SRR1512602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605985
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55890.21446784996843804No Hit
GTATCAACGCAGAGTACTTTTTTTT51130.19620220377323735No Hit
CTGTAGGACGTGGAATATGGCAAGA49800.1910985673363431No Hit
GTCCTAAAGTGTGTATTTCTCATTT48250.18515072036101513No Hit
CTTTAGGACGTGAAATATGGCGAGG41200.1580976099248461No Hit
TATCAACGCAGAGTACTTTTTTTTT32300.12394545632457593No Hit
GGTATCAACGCAGAGTACTTTTTTT30020.11519636528989999No Hit
GTCCTACAGTGTGCATTTCTCATTT28570.10963225037749641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10700.012.6620174
TTAACGT550.003132105612.0582984
AGGACCG1052.8194518E-711.7301815
GTATTAC2250.011.5939951
GTGTAGG4450.011.4987971
GGACCGC752.1271466E-411.3694726
TGTAGGA33600.011.1946432
GGCGAGG13550.011.1078919
CGGTTTC1803.6379788E-1211.05621213
GTAGGAC32600.011.0439653
AGGACCT21200.011.0385435
GTCCTAA15350.010.91566851
AGGACGT32200.010.7101655
CTGTAGG31300.010.6423121
GGACCTG20500.010.5837766
GGACGTG31600.010.553946
GTCCTAG4100.010.5226921
TAGGACA10350.010.4355454
GACGTGG16350.010.4309087
TCCAACG3100.010.37923618
Produced by FastQC (version 0.11.5)