FastQCFastQC Report
Thu 26 May 2016
SRR1512602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605985
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64220.24643273081003922No Hit
GTCCTACAGTGGACATTTCTAAATT60270.23127531432452605No Hit
CTGTAGGACGTGGAATATGGCAAGA56830.21807493135992725No Hit
GTCCTAAAGTGTGTATTTCTCATTT51170.19635569659840713No Hit
CTTTAGGACGTGAAATATGGCGAGG48160.18480536150438318No Hit
TATCAACGCAGAGTACTTTTTTTTT42890.16458268178826818No Hit
GGTATCAACGCAGAGTACTTTTTTT41430.15898019366957214No Hit
GTCCTACAGTGTGCATTTCTCATTT32300.12394545632457593No Hit
CTGTAGGACCTGGAATATGGCGAGA26340.10107502537428266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGA500.001484673613.3181651
TAGGACC11750.013.0195334
CCAACGA1800.012.661818519
TCCAACG2500.012.53592318
AATACCG550.00306408912.0937075
GCGCCAC1001.9345844E-611.39826213
TGTAGGA34750.011.2928272
AGGACCT21100.011.2134945
GTAGGAC33850.011.1439513
ACCGCCC600.005878261811.0831328
GTTCGAT600.00587910211.08291911
TGTTGCG600.00589087111.07994216
GGCGAGG12550.011.04756419
CTAGGAC4050.011.0268193
CTGTAGG34850.011.0006561
GGACCTG20400.010.9912626
AGGACGT30800.010.7979535
GGACGTG30150.010.7141416
CCGTCCA909.523437E-510.55516059
TTAGGAC17800.010.4093123
Produced by FastQC (version 0.11.5)