FastQCFastQC Report
Thu 26 May 2016
SRR1512601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448182
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58910.24062753504437168No Hit
GTCCTAAAGTGTGTATTTCTCATTT50100.20464164837418133No Hit
GTATCAACGCAGAGTACTTTTTTTT49800.20341624928212035No Hit
CTGTAGGACGTGGAATATGGCAAGA49570.20247677664487362No Hit
CTTTAGGACGTGAAATATGGCGAGG43390.17723355534841773No Hit
TATCAACGCAGAGTACTTTTTTTTT30070.12282583566091082No Hit
GGTATCAACGCAGAGTACTTTTTTT29490.12045673074959297No Hit
GTCCTACAGTGTGCATTTCTCATTT29010.11849609220229541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.012.3548654
GGTATCA14900.012.1857051
GGCGAGG11600.011.74803819
GCACCGT600.0059910711.05481156
CTAGGAC3500.010.558693
AGGACGT32850.010.5283925
AAAGGCG1451.5035766E-810.4557825
CTCGAAC1451.5355909E-810.4416318
GGACGTG32650.010.4187556
TGGCGAG29200.010.40251918
AGGCGTG1951.6370905E-1110.2044427
TTAGGAC17200.010.1919383
GACGTGG17700.010.1179647
TAGGCCG750.002705702810.1072575
TGTAGGA34250.010.042912
GTCCTAC29000.09.9967371
CAGGAGT3050.09.9415654
GACGTGA16400.09.9377757
TGTCGAG2002.7284841E-119.93586418
AATGTCC8500.09.8974788
Produced by FastQC (version 0.11.5)