FastQCFastQC Report
Thu 26 May 2016
SRR1512601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448182
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62540.25545486405830936No Hit
GTATCAACGCAGAGTACTTTTTTTT62080.2535759187838159No Hit
CTGTAGGACGTGGAATATGGCAAGA57390.2344188463112628No Hit
GTCCTAAAGTGTGTATTTCTCATTT53360.21795765184124383No Hit
CTTTAGGACGTGAAATATGGCGAGG50500.20627551383026263No Hit
TATCAACGCAGAGTACTTTTTTTTT41650.17012624061446413No Hit
GGTATCAACGCAGAGTACTTTTTTT41280.16861491506758894No Hit
GTCCTACAGTGTGCATTTCTCATTT31650.12927960421243193No Hit
CTGAAGGACCTGGAATATGGCGAGA27110.1107352312859093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9800.012.7979424
TATACCG701.08957734E-412.2164675
TACGGCG550.003076958512.08689316
GGCGAGG12850.011.89804119
TTAGACA2150.010.6063274
AGGACGT31150.010.5540515
GGACGTG30800.010.5490756
GGTATCA17150.010.5406611
AGGACCT19700.010.5145685
TTAGGAC18150.010.46997453
TGTAGGA34250.010.4031052
GTAGGAC33100.010.3052543
GACGTGG16600.010.18697
GCCGGTT1409.6986696E-810.178308511
GACGTGA15500.010.1743477
TAGGACG33700.010.0653894
CAGCGAC856.5973913E-410.0585649
TCCAACG1701.5697879E-910.05548318
CTGTAGG33650.010.0091361
TGGCGAG30700.09.99179518
Produced by FastQC (version 0.11.5)