FastQCFastQC Report
Thu 26 May 2016
SRR1512600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993374
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT48210.24185125320185774No Hit
CTGTAGGACGTGGAATATGGCAAGA43100.21621632468367702No Hit
GTCCTAAAGTGTGTATTTCTCATTT41760.20949405380024022No Hit
GTATCAACGCAGAGTACTTTTTTTT38380.19253787798978014No Hit
GTACATGGGAAGCAGTGGTATCAAC37850.189879069356779No Hit
CTTTAGGACGTGAAATATGGCGAGG36270.18195280965839827No Hit
CCCATGTACTCTGCGTTGATACCAC29050.14573281280883565No Hit
GGTATCAACGCAGAGTACTTTTTTT23140.1160845882408419No Hit
TATCAACGCAGAGTACTTTTTTTTT22100.11086730337608497No Hit
GTCCTACAGTGTGCATTTCTCATTT20000.10033240124532578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2400.012.22416418
TAACGCT550.00313166912.0582924
TCTACGC550.00313166912.0582923
TAGGACC7700.011.9352494
CCAACGA1603.6379788E-1211.83222119
GCCGGTT1157.3752744E-811.53081911
CGTGCAC1002.0024963E-611.36523310
TGTCGAG1002.02079E-611.35664318
GGCGAGG11400.011.20947419
ACTGTTC4700.010.860528
AAATGTC6900.010.8480177
CTAGTAC700.001525707710.8278543
TAGTACT1701.4551915E-1010.58900454
GTCCTAC22600.010.527691
TCAATAC1355.9188096E-810.52708153
TAGGACA7050.010.4822854
TTAGGAC15700.010.3795673
TATGTCG1303.9597762E-710.20832116
TTTAGAA8000.010.1849512
ATAGGAC2800.010.15111353
Produced by FastQC (version 0.11.5)