FastQCFastQC Report
Thu 26 May 2016
SRR1512600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993374
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51130.2564997837836753No Hit
CTGTAGGACGTGGAATATGGCAAGA48810.24486122523921755No Hit
GTATCAACGCAGAGTACTTTTTTTT48460.24310540821742432No Hit
GTCCTAAAGTGTGTATTTCTCATTT44170.22158410815030197No Hit
CTTTAGGACGTGAAATATGGCGAGG43660.21902563191854613No Hit
GTACATGGGAAGCAGTGGTATCAAC43640.21892529951730083No Hit
CCCATGTACTCTGCGTTGATACCAC33540.1682574368884113No Hit
GGTATCAACGCAGAGTACTTTTTTT32490.1629899858230317No Hit
TATCAACGCAGAGTACTTTTTTTTT31750.15927768697695466No Hit
CTGAAGGACCTGGAATATGGCGAGA23590.11834206726886173No Hit
GTCCTACAGTGTGCATTTCTCATTT22560.11317494860472745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7950.013.029084
TCCAACG2250.012.2395318
TGCGCCA1552.5465852E-1111.64399112
ACCGTCC752.0748578E-411.39971358
CCAACGA1701.2732926E-1111.17142419
GTCGTAA600.005816528611.0986541
GCGCCAC1201.2784403E-711.08166613
GTAGGCC951.3560826E-511.0033133
GGACGTG25250.010.9869666
AGGACGT26250.010.8604145
AATGTCC7700.010.856878
CGGTTTC1752.0008883E-1110.85550813
GACGTGA12900.010.825317
TGTCCAC7950.010.75444710
GGCGAGG11050.010.6558219
TAGAAAT7800.010.5993464
ATTTAGA7650.010.5701461
CTAGGAC3300.010.3667593
ATAGGAC2750.010.3667583
GTGCGCC1659.295036E-1010.36311611
Produced by FastQC (version 0.11.5)