FastQCFastQC Report
Thu 26 May 2016
SRR1512599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241170
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37240.16616320939509274No Hit
GTATCAACGCAGAGTACTTTTTTTT36250.16174587380698474No Hit
GTCCTAAAGTGTGTATTTCTCATTT34150.15237576801402838No Hit
CTGTAGGACGTGGAATATGGCAAGA33300.1485831061454508No Hit
CTTTAGGACGTGAAATATGGCGAGG29960.13368017597951068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7500.013.3909434
ACTGCGC600.006112804611.0249018
AGGACCT14900.010.9372655
TGTAGGA22800.010.596732
GTCCTAC20050.010.4695621
GTAGGAC22250.010.3902583
TGGCGAG21000.010.27416318
GGCGAGG9950.010.27351919
GTCCTAA10700.010.23151
GGACCTG14800.010.17892556
GTCTATA2102.1827873E-1110.0437661
TCCAACG1701.6680133E-910.01969518
GTATAAG2950.09.8735321
GTCTTAT2450.09.8387911
TCCTACA22750.09.8287242
TAGACAG3400.09.7533925
CTAACAC1752.6957423E-99.7454293
CTGTAGG21450.09.7394091
GTACTAA1551.8994251E-79.7197731
TAGGACT4050.09.5916954
Produced by FastQC (version 0.11.5)