FastQCFastQC Report
Thu 26 May 2016
SRR1512599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241170
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44090.19672760210068846No Hit
GTCCTACAGTGGACATTTCTAAATT40200.17937059660802168No Hit
CTGTAGGACGTGGAATATGGCAAGA38470.17165141421668148No Hit
CTTTAGGACGTGAAATATGGCGAGG35490.15835478790096244No Hit
GTCCTAAAGTGTGTATTTCTCATTT35180.1569715818077165No Hit
TATCAACGCAGAGTACTTTTTTTTT28980.12930745994279771No Hit
GGTATCAACGCAGAGTACTTTTTTT27620.12323920095307361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1800.013.18940818
TAGGACC7600.012.1271374
GGCGAGG9800.012.11245219
ACTGCGT802.8670998E-511.87523658
GCCGGTT1302.6157068E-911.69254111
CCGGTTT1303.2716343E-810.96077912
TGGCGAG20650.010.94497318
CGAGACG700.001496606210.85299918
CCAACGA1053.484667E-610.85275719
TTAGGAC14800.010.7856853
GGACGTG21650.010.707396
AGGACGT21950.010.6925675
CGTTTCC909.5467236E-510.55246814
ACTGTTC5200.010.2309738
AGGACCT15050.010.2281725
GTATTAT2053.6379788E-1210.2083141
TAGGACG23650.010.1645814
CACACCG750.002643504210.13557155
ACCGAGT750.002647913810.1335358
ATGGCGA22800.010.12113517
Produced by FastQC (version 0.11.5)