FastQCFastQC Report
Thu 26 May 2016
SRR1512598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576987
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67130.2604980157059387No Hit
CTGTAGGACGTGGAATATGGCAAGA59690.2316270900862131No Hit
GTCCTAAAGTGTGTATTTCTCATTT56130.21781250739720454No Hit
CTTTAGGACGTGAAATATGGCGAGG49170.19080422214004183No Hit
GTATCAACGCAGAGTACTTTTTTTT39280.15242606966973446No Hit
GTCCTACAGTGTGCATTTCTCATTT37510.1455575833327836No Hit
CTGAAGGACCTGGAATATGGCGAGA26740.10376459019777748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG400.00541707414.19518419
CCGTGCG400.005457829714.17915259
TCCAACG2900.013.70222318
TAGGACC12200.012.8910824
CCAACGA2250.012.19734419
GTCCTAG3750.012.0505921
AGAACCG1052.8201976E-711.7299235
TCTACGG658.2092604E-411.6609712
CGGTTTC2300.011.53714813
CCGGTTT2150.011.45646412
GGCGAGG14700.011.394773519
GACTGCG752.1267869E-411.3696697
TATTCCG752.1279434E-411.3690035
TATACCG600.005997730411.0531975
ACCGCTC600.00611632311.024158
CTACACT5400.010.877754
GTCCTAT3350.010.7915751
TGTAGGA37000.010.6268472
GTATAGG2950.010.5527791
GTCCTAC33500.010.5217861
Produced by FastQC (version 0.11.5)