FastQCFastQC Report
Thu 26 May 2016
SRR1512598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576987
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72500.28133630476211174No Hit
CTGTAGGACGTGGAATATGGCAAGA69530.2698112175187535No Hit
GTCCTAAAGTGTGTATTTCTCATTT60640.23531356580378557No Hit
CTTTAGGACGTGAAATATGGCGAGG58380.22654363409671838No Hit
GTATCAACGCAGAGTACTTTTTTTT52740.20465760983660375No Hit
GTCCTACAGTGTGCATTTCTCATTT39590.1536290249038897No Hit
TATCAACGCAGAGTACTTTTTTTTT35530.13787419183721145No Hit
GGTATCAACGCAGAGTACTTTTTTT34180.13263551581750313No Hit
CTGAAGGACCTGGAATATGGCGAGA34000.13193702568154206No Hit
GTACATGGGAAGCAGTGGTATCAAC31500.12223577379319338No Hit
CTGTAGGACCTGGAATATGGCGAGA30900.11990747333998969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA400.005277631314.2511067
TAGGACC10800.013.9027374
CGGCGAG550.003079218312.08574818
TCCAACG2850.011.66168618
AGGACCT23150.011.3711445
CGTAGAC600.00586301411.0869933
AGCCGTC600.005891887511.07967815
GGCGAGG16450.010.91002319
GGACCTG23300.010.8059796
AAGTCGT2300.010.7399647
TACGACA803.761001E-410.6910284
GTAGGAC37050.010.6188893
GTATAAG3250.010.5369851
TGTAGGA37800.010.53392
CCAACGA1909.094947E-1210.49531519
AAATGTC7800.010.4751717
GACGTGA18900.010.3553017
GGACGTG34750.010.3076756
GTCTTAC2500.010.27356151
AGGACGT36050.010.2282625
Produced by FastQC (version 0.11.5)