FastQCFastQC Report
Thu 26 May 2016
SRR1512597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052856
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65500.31906767936961966No Hit
GTCCTAAAGTGTGTATTTCTCATTT62140.30270023810730023No Hit
CTGTAGGACGTGGAATATGGCAAGA58350.2842381540643864No Hit
CTTTAGGACGTGAAATATGGCGAGG52660.2565206716886133No Hit
GTATCAACGCAGAGTACTTTTTTTT38330.1867154832097332No Hit
GTCCTACAGTGTGCATTTCTCATTT32540.15851087460591487No Hit
CTGAAGGACCTGGAATATGGCGAGA28730.13995136531739197No Hit
GTACATGGGAAGCAGTGGTATCAAC26720.13016012813368302No Hit
TATCAACGCAGAGTACTTTTTTTTT24230.11803068505535702No Hit
GGTATCAACGCAGAGTACTTTTTTT23930.11656930637122137No Hit
GTCCTTCAGTGTGCATTTCTCATTT23740.11564376653793544No Hit
TTTCTAAATTTTCCACCTTTTTCAG23620.11505921506428118No Hit
ATTTAGAAATGTCCACTGTAGGACG23230.11315942277490482No Hit
CTGTAGGACCTGGAATATGGCGAGA21770.1060473798454446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1950.014.07380518
TAGGACC9300.012.9374074
TTGCGAG854.1188596E-612.2467818
AATGTCC8100.011.5505838
GTCCTAT4000.011.540941
GGCGAGG16600.011.11857219
GTGTAGG3550.011.0250251
AAATGTC8250.011.0243847
GTATTGG2550.011.0194831
GACGTGG17350.010.921137
CACCTTT9500.010.87771814
ATTGCGA1053.5533812E-610.8349517
TGGCGAA1408.87303E-910.81533918
AGGACGT37150.010.8126825
CCACCTT9400.010.78802413
TATGTCG1502.2082531E-910.74439516
GTATTAG2250.010.7046391
CCAACGT1159.248506E-710.69961319
GGACGTG36350.010.6857726
TGTCCAC9150.010.6619410
Produced by FastQC (version 0.11.5)