FastQCFastQC Report
Thu 26 May 2016
SRR1512597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052856
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68940.33582482161437527No Hit
CTGTAGGACGTGGAATATGGCAAGA67350.32807951458845624No Hit
GTCCTAAAGTGTGTATTTCTCATTT67020.32647199803590704No Hit
CTTTAGGACGTGAAATATGGCGAGG62990.3068408110456846No Hit
GTATCAACGCAGAGTACTTTTTTTT48750.23747403617204518No Hit
CTGAAGGACCTGGAATATGGCGAGA34240.16679202048268363No Hit
GTCCTACAGTGTGCATTTCTCATTT34200.16659716999146557No Hit
GTACATGGGAAGCAGTGGTATCAAC33440.16289501065832188No Hit
TATCAACGCAGAGTACTTTTTTTTT33110.16128749410577264No Hit
GGTATCAACGCAGAGTACTTTTTTT31090.15144754429925916No Hit
ATTTAGAAATGTCCACTGTAGGACG28500.13883097499288796No Hit
TTTCTAAATTTTCCACCTTTTTCAG28490.13878226237008345No Hit
CTGTAGGACCTGGAATATGGCGAGA27070.1318650699318413No Hit
GTCCTTCAGTGTGCATTTCTCATTT25480.12411976290592228No Hit
CCCATGTACTCTGCGTTGATACCAC24280.11827424816937962No Hit
GAATATGGCAAGAAAACTGAAAATC23820.11603346752037161No Hit
GGAATATGGCGAGAAAACTGAAAAT21080.10268620887193257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1052.0059815E-812.66161719
TAGGACC9550.012.5369744
ATTGCGA550.003078201612.08608917
TCCAACG1650.012.08608818
GGCGAGG17100.011.71754819
CGAAATC2000.011.39934313
CTAAGAC1451.2132659E-911.1405463
CCATTAG1201.2529927E-711.09783651
ACCTTTT9450.011.05513915
CACCTTT9350.010.97022314
AGGACGT37700.010.7120635
CTAGGAC4350.010.7036613
GACGGGA803.7703975E-410.6879257
GGACGTG37250.010.6350636
TGTAGGA34700.010.6247132
ACGAAAT2150.010.60403912
CCACCTT9600.010.48897813
TCGAACT1553.6870915E-910.415219
TAGAAAT9900.010.366074
GACGTGG18400.010.2748657
Produced by FastQC (version 0.11.5)