FastQCFastQC Report
Thu 26 May 2016
SRR1512596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981023
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66550.3359375433803646No Hit
GTCCTAAAGTGTGTATTTCTCATTT62010.3130200911347319No Hit
CTGTAGGACGTGGAATATGGCAAGA60340.30459010319415775No Hit
CTTTAGGACGTGAAATATGGCGAGG53480.26996152997718853No Hit
GTATCAACGCAGAGTACTTTTTTTT40090.20237018954348338No Hit
GTCCTACAGTGTGCATTTCTCATTT34040.1718304128725411No Hit
CTGAAGGACCTGGAATATGGCGAGA28840.145581348626442No Hit
GTCCTTCAGTGTGCATTTCTCATTT25020.12629838219949996No Hit
CTGTAGGACCTGGAATATGGCGAGA24940.12589455044186765No Hit
GGTATCAACGCAGAGTACTTTTTTT23790.12008946892590343No Hit
TATCAACGCAGAGTACTTTTTTTTT23520.11872653674389444No Hit
ATTTAGAAATGTCCACTGTAGGACG23280.11751504147099756No Hit
TTTCTAAATTTTCCACCTTTTTCAG22690.1145367822584594No Hit
GTACATGGGAAGCAGTGGTATCAAC22400.11307289213704232No Hit
GAATATGGCAAGAAAACTGAAAATC21380.10792403722723058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1006.9667294E-1014.19875419
TCCAACG1550.014.0432318
CCCGTTT1001.0490112E-813.26192512
TAGGACC11100.013.2291324
GCACCGT701.11945046E-412.1808656
GCTCCGT701.11945046E-412.1808656
GCGCCAT550.003137927312.05507211
ACCTTTT9300.011.41941315
GGCGAGG15100.011.40915419
CCGGTTT1251.9117579E-811.36736512
CCACCTT9350.011.35257713
AATGTCC8150.011.2446998
CACCTTT10150.011.02362914
TGTAGGA37450.010.87885952
ATAGGAC3050.010.8723623
CTAGTAC1053.580646E-610.8279853
GTCCTAA19400.010.809371
ATGTCCA8950.010.7728539
AATCCCG1855.456968E-1210.74500419
GACGTGG19400.010.7437197
Produced by FastQC (version 0.11.5)