FastQCFastQC Report
Thu 26 May 2016
SRR1512596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981023
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73880.37293862817342355No Hit
CTGTAGGACGTGGAATATGGCAAGA72760.3672849835665714No Hit
GTCCTAAAGTGTGTATTTCTCATTT68210.34431705235123466No Hit
CTTTAGGACGTGAAATATGGCGAGG62950.3177651142869114No Hit
GTATCAACGCAGAGTACTTTTTTTT51320.2590580725211166No Hit
GTCCTACAGTGTGCATTTCTCATTT38450.1940916385120213No Hit
CTGAAGGACCTGGAATATGGCGAGA36200.18273387032861305No Hit
GGTATCAACGCAGAGTACTTTTTTT33600.16960933820556348No Hit
TATCAACGCAGAGTACTTTTTTTTT33500.16910454850852313No Hit
CTGTAGGACCTGGAATATGGCGAGA29130.14704523874785905No Hit
ATTTAGAAATGTCCACTGTAGGACG27780.1402305778378141No Hit
TTTCTAAATTTTCCACCTTTTTCAG26900.13578842850385886No Hit
GTCCTTCAGTGTGCATTTCTCATTT26590.13422358044303373No Hit
GTACATGGGAAGCAGTGGTATCAAC25940.13094244741227135No Hit
GAATATGGCAAGAAAACTGAAAATC24640.12438018135074655No Hit
GGAATATGGCGAGAAAACTGAAAAT20660.10428955140853993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11650.013.2950264
CCAACGA1001.4515354E-712.34453619
GGCGAGG15900.011.82495519
CCGTGCA658.027722E-411.6918579
TAGAAAT10650.011.24212554
ATACGGA600.005869476611.0851466
GGACCTG22850.010.8113966
TATGTCG1502.1482265E-910.76217616
AGGACCT23850.010.75783355
ACTGTTC6400.010.6870888
CACCTTT9600.010.6843914
GACGTGA19650.010.5884487
TTAGGAC22400.010.5201083
AAATGTC9850.010.5133477
CCACCTT9500.010.49800513
GGACGTG36200.010.4464596
AGGACGT37100.010.4248545
ATTTAGA10300.010.3469451
TGGCGAG37950.010.3342918
GAAATGT10400.010.3238036
Produced by FastQC (version 0.11.5)