FastQCFastQC Report
Thu 26 May 2016
SRR1512595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757283
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38560.21942965361868294No Hit
GTCCTAAAGTGTGTATTTCTCATTT36590.20821916560963713No Hit
CTGTAGGACGTGGAATATGGCAAGA34930.19877276454617726No Hit
GTATCAACGCAGAGTACTTTTTTTT34920.19871585851567447No Hit
GTACATGGGAAGCAGTGGTATCAAC33260.1892694574522146No Hit
CTTTAGGACGTGAAATATGGCGAGG30940.17606725837557183No Hit
CCCATGTACTCTGCGTTGATACCAC23920.13611922496262696No Hit
GGTATCAACGCAGAGTACTTTTTTT22940.13054243397335547No Hit
TATCAACGCAGAGTACTTTTTTTTT22570.12843691084475295No Hit
GTCCTACAGTGTGCATTTCTCATTT19000.1081214579552639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.013.9168174
CTAGGAC2350.012.497313
TAAGACG550.003133584512.0571764
AATCCCG951.0880176E-611.955235519
GCTAAAC855.475549E-511.1456093
TGAACCG700.001526728310.8268535
GTCTAAC650.007046825710.8157711
GTCCTAA11000.010.6823291
TGTAGGA23700.010.4331362
ATTTAGA5900.010.3836641
AGGACCT13600.010.3790515
AAATGTC6150.010.3207197
GTATTAA2153.6379788E-1210.2767781
GGACCTG13000.010.27516
GTTCTAA1903.8198777E-1010.0432161
ATAAGAC1804.1472958E-1010.0000883
TAGAAAT6650.09.9721014
GAAATGT6400.09.9175666
GTCCTAC18950.09.910721
AATGTCC6200.09.9092138
Produced by FastQC (version 0.11.5)