FastQCFastQC Report
Thu 26 May 2016
SRR1512595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757283
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47370.2695638664916237No Hit
GTCCTACAGTGGACATTTCTAAATT41150.23416831551890047No Hit
CTGTAGGACGTGGAATATGGCAAGA39810.22654290743152924No Hit
GTACATGGGAAGCAGTGGTATCAAC39260.22341307575387687No Hit
GTCCTAAAGTGTGTATTTCTCATTT39000.22193351896080482No Hit
CTTTAGGACGTGAAATATGGCGAGG36560.20804844751812884No Hit
GGTATCAACGCAGAGTACTTTTTTT30880.17572582219255523No Hit
TATCAACGCAGAGTACTTTTTTTTT30640.17436007746048873No Hit
CCCATGTACTCTGCGTTGATACCAC28190.16041809998730996No Hit
GTCCTACAGTGTGCATTTCTCATTT20800.11836454344576258No Hit
CTGAAGGACCTGGAATATGGCGAGA20370.11591758413414344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG456.752663E-414.7807454
GCGCTAC400.00523867114.2666711
TAGGACC6600.012.669214
TATGTCG1052.0027073E-812.6627216
GTCCTAG2100.011.7756641
GTCTAAC752.0517057E-411.4133361
GGCGAGG11100.011.37901319
CAAGTCG1104.9634036E-711.2282496
CTAGGAC2300.011.1544133
AGACCGT600.005872374411.0842976
ACCGTGC700.00149190610.856858
TTAGGAC14750.010.8225123
ACCTTTT6600.010.79270615
GACGTGA12100.010.756527
TGTCGAG1158.8525667E-710.735785518
TGGCGAG22850.010.72313618
AGGACCT15450.010.5166745
TGTAGGA21900.010.4998252
TAGGACA7000.010.4520994
GGACGTG22000.010.3645376
Produced by FastQC (version 0.11.5)