FastQCFastQC Report
Thu 26 May 2016
SRR1512594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552927
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT93850.3676172487501601No Hit
CTGTAGGACGTGGAATATGGCAAGA82850.324529451880136No Hit
GTCCTAAAGTGTGTATTTCTCATTT72530.28410526427116795No Hit
CTTTAGGACGTGAAATATGGCGAGG57770.22628927501648108No Hit
GTCCTACAGTGTGCATTTCTCATTT50640.19836054849982002No Hit
GTACATGGGAAGCAGTGGTATCAAC44250.17333045559077875No Hit
CCCATGTACTCTGCGTTGATACCAC36840.1443049487901534No Hit
GTATCAACGCAGAGTACTTTTTTTT36530.1430906563329073No Hit
CTGTAGGACCTGGAATATGGCGAGA36360.14242475401764326No Hit
CTGAAGGACCTGGAATATGGCGAGA34530.13525651144744835No Hit
GTCCTTCAGTGTGCATTTCTCATTT29300.11477022257197327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14950.013.1177844
TCCAACG3350.012.71294218
ATGCGCT550.003135891912.05633611
CCAACGA2200.012.04685119
GGACGTC658.21211E-411.6604816
GTCCTAT3800.011.6197291
GTCCTAC44300.011.4850041
GTGTAGG4950.011.3529781
TGTAGGA48900.011.1986932
ACCTTTT9900.011.10713715
GGCGAGG16600.011.00493419
TCCTACA49350.011.00058652
GTAGGAC47800.011.0000933
CATTTCT60500.010.96786214
CTGTAGG45300.010.899181
GTCCTAG3700.010.84888651
TGCGAGA1053.6234615E-610.81758119
TTGCGAG700.001539859310.81609118
AGGACCT28650.010.8132055
ATTTCTA32350.010.81263115
Produced by FastQC (version 0.11.5)