FastQCFastQC Report
Thu 26 May 2016
SRR1512594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552927
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT99340.38912197646074487No Hit
CTGTAGGACGTGGAATATGGCAAGA96840.37932929535392124No Hit
GTCCTAAAGTGTGTATTTCTCATTT77610.3040039922802336No Hit
CTTTAGGACGTGAAATATGGCGAGG70020.274273412439917No Hit
GTCCTACAGTGTGCATTTCTCATTT57670.22589756777220815No Hit
GTACATGGGAAGCAGTGGTATCAAC53950.21132605828525453No Hit
GTATCAACGCAGAGTACTTTTTTTT49070.19221074476473476No Hit
CTGTAGGACCTGGAATATGGCGAGA43130.16894333445492174No Hit
CTGAAGGACCTGGAATATGGCGAGA41320.1618534333335814No Hit
CCCATGTACTCTGCGTTGATACCAC39900.15629119046490558No Hit
GGTATCAACGCAGAGTACTTTTTTT31290.12256519673300491No Hit
TATCAACGCAGAGTACTTTTTTTTT31140.12197763586659549No Hit
GTCCTTCAGTGTGCATTTCTCATTT30140.11806056342386602No Hit
ATTTAGAAATGTCCACTGTAGGACG29680.11625871010021045No Hit
TTTCTAAATTTTCCACCTTTTTCAG29030.11371261301243632No Hit
GAATATGGCAAGAAAACTGAAAATC28280.11077480868038922No Hit
TTGTAGAACAGTGTATATCAATGAG28080.10999139419184334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14400.013.7249114
ACCGTGC951.0413696E-611.9992958
CCAACGA2400.011.87127919
GGCGAGG19700.011.47356119
TCCAACG3650.011.4486918
ACTGCGT752.0759429E-411.399338
TAGGACT4850.011.3630514
AGGACGT47200.011.0924345
AAGGCGT1803.6379788E-1211.0846376
ATGCCGC600.005872271511.0846366
TTAGGAC26800.011.0264143
GGACGTG46900.011.0002276
CTAGGAC4250.010.9550893
TGTAGGA49050.010.9256892
AGGACCT29100.010.8408245
GGACCTG28750.010.8395366
GTAGGAC48950.010.7150713
TAGAAAT10750.010.6951184
CTGTAGG47600.010.6898781
GACGTGA23750.010.680217
Produced by FastQC (version 0.11.5)