FastQCFastQC Report
Thu 26 May 2016
SRR1512593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174769
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46760.2150113414344236No Hit
GTCCTAAAGTGTGTATTTCTCATTT42940.19744625751056782No Hit
GTATCAACGCAGAGTACTTTTTTTT40880.18797398712231045No Hit
CTGTAGGACGTGGAATATGGCAAGA40490.18618069321385397No Hit
CTTTAGGACGTGAAATATGGCGAGG35730.16429331115166715No Hit
GTCCTACAGTGTGCATTTCTCATTT26490.12180604008977505No Hit
GGTATCAACGCAGAGTACTTTTTTT24340.11191993264572006No Hit
TATCAACGCAGAGTACTTTTTTTTT23610.10856325430425025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC400.00535523914.21963114
TAGGACC9050.013.0875314
AGGACCT18200.011.1934345
TACACCG855.4675038E-511.1474975
TGTAGGA27950.010.6452082
GTATAAT2550.010.6066711
AATCCCG1355.999755E-810.51749619
GGACCTG18250.010.4880516
GTAGGAC26850.010.481383
CTGTAGG26300.010.474481
GTTTAGA2700.010.3884261
GCAGGAC2600.010.2044833
GTCCTAA13300.010.1680491
TGGCGAG25450.010.11499718
ATAGGAC3000.010.1072983
CACCTTT6500.010.06312414
GTATTAG1405.129823E-710.0174111
GTCCTAC24200.010.0174111
TAGAAAT7250.09.802114
TAGGACT3000.09.7912194
Produced by FastQC (version 0.11.5)