##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512593_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2174769 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.025895623857064 31.0 25.0 33.0 2.0 34.0 2 27.570710728357817 31.0 26.0 34.0 16.0 34.0 3 28.636750845722005 31.0 28.0 34.0 16.0 34.0 4 32.38532184337739 35.0 33.0 37.0 22.0 37.0 5 32.66777850888991 35.0 35.0 37.0 25.0 37.0 6 32.87501799041645 37.0 35.0 37.0 26.0 37.0 7 32.83735881833887 37.0 35.0 37.0 25.0 37.0 8 32.845911910644304 37.0 35.0 37.0 25.0 37.0 9 34.27697562361796 39.0 35.0 39.0 25.0 39.0 10 34.175980989245296 39.0 35.0 39.0 25.0 39.0 11 34.23039274516052 39.0 35.0 39.0 25.0 39.0 12 34.1440001213922 39.0 35.0 39.0 25.0 39.0 13 34.10767856264275 39.0 35.0 39.0 25.0 39.0 14 35.15749810669547 40.0 36.0 41.0 24.0 41.0 15 35.07714888339865 40.0 36.0 41.0 22.0 41.0 16 34.93392401675764 40.0 36.0 41.0 19.0 41.0 17 34.86330640173738 40.0 36.0 41.0 19.0 41.0 18 34.81436373242399 40.0 35.0 41.0 18.0 41.0 19 34.81518634852713 40.0 35.0 41.0 18.0 41.0 20 34.612442976702354 40.0 35.0 41.0 18.0 41.0 21 34.57454239967555 40.0 35.0 41.0 18.0 41.0 22 34.47430186838234 40.0 35.0 41.0 15.0 41.0 23 34.312519628521464 39.0 34.0 41.0 10.0 41.0 24 34.181725967217666 39.0 34.0 41.0 2.0 41.0 25 34.040988261282 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -2.6892494849445363 1101 2 -4.222491819079789 1101 3 -5.006109739978076 1101 4 -5.797950911579935 1101 5 -6.089723415361291 1101 6 -6.04345018913947 1101 7 -6.088770892394898 1101 8 -6.186851918084024 1101 9 -6.344154624200694 1101 10 -7.018754112527933 1101 11 -6.9564711429940616 1101 12 -6.89588102550443 1101 13 -6.957456888532121 1101 14 -8.193474773744452 1101 15 -8.033844342696778 1101 16 -8.263725036042928 1101 17 -8.439805304957332 1101 18 -8.37058599666722 1101 19 -8.388340748775605 1101 20 -8.517275630106557 1101 21 -8.819207400102087 1101 22 -8.524912529216571 1101 23 -8.95158939568164 1101 24 -8.835169761399499 1101 25 -9.46441077829715 1102 1 0.3926256352010249 1102 2 -0.3805989062149351 1102 3 -1.107116950946974 1102 4 -1.6313413187113284 1102 5 -1.4483776956726189 1102 6 -1.373343745760998 1102 7 -1.5091037682437403 1102 8 -1.434101422972553 1102 9 -1.6367563399910594 1102 10 -1.9102645713386792 1102 11 -1.8396208119511286 1102 12 -1.8416512311936302 1102 13 -2.0643146853643017 1102 14 -2.383346335346154 1102 15 -2.3951402606535623 1102 16 -2.3591306698079606 1102 17 -2.2850430702275517 1102 18 -2.5631215485768095 1102 19 -2.6001939186066636 1102 20 -2.6478317196423795 1102 21 -2.6881206583602797 1102 22 -2.6532731402489524 1102 23 -2.811187852453827 1102 24 -2.9641370236901103 1102 25 -2.8507685013655433 1103 1 2.4374865245574675 1103 2 0.781809473674933 1103 3 0.13708091982602966 1103 4 0.08338117662346178 1103 5 -0.2469445951936322 1103 6 -0.28053081847519223 1103 7 -0.5610524065188045 1103 8 -0.4051742632891262 1103 9 -0.5968688066323153 1103 10 -0.810272884152738 1103 11 -0.784329490815658 1103 12 -0.8736989209479447 1103 13 -0.8146135166973067 1103 14 -0.9391661651341181 1103 15 -0.9737368426537429 1103 16 -1.0473263305190272 1103 17 -1.0278503362003377 1103 18 -0.9219484671886207 1103 19 -0.9990051145339578 1103 20 -1.0745574885060165 1103 21 -1.1607700668605574 1103 22 -1.2951929416586907 1103 23 -1.5563502963065048 1103 24 -1.5098048293078534 1103 25 -1.4301712830051159 1104 1 1.9774478409942553 1104 2 1.0311920077002128 1104 3 -0.029189048321658362 1104 4 -0.5687631448390285 1104 5 -0.6816396338368911 1104 6 -0.8495242868035682 1104 7 -0.9628230913770679 1104 8 -0.9285671053185922 1104 9 -1.0967571354674561 1104 10 -1.1412440504900445 1104 11 -1.1791008285724018 1104 12 -1.3601689097841856 1104 13 -1.3938358958002581 1104 14 -1.7598310065509892 1104 15 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0.5824766725552664 2316 25 0.6874971992192087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120572.0 3 12.0 4 1086.0 5 4328.0 6 4983.0 7 4549.0 8 4060.0 9 3911.0 10 3838.0 11 3684.0 12 3881.0 13 3940.0 14 3929.0 15 4053.0 16 4209.0 17 4492.0 18 4975.0 19 5999.0 20 7556.0 21 9154.0 22 11265.0 23 12903.0 24 15022.0 25 17096.0 26 19633.0 27 22175.0 28 25244.0 29 30216.0 30 37387.0 31 47818.0 32 60777.0 33 81048.0 34 111805.0 35 159679.0 36 240186.0 37 317510.0 38 761794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19065217889668 19.802710872022043 11.916754991856848 25.08988195722443 2 14.826148824126829 20.03269847179803 41.49844341265877 23.642709291416374 3 18.955155665597477 24.62116779375679 29.533477755673154 26.89019878497258 4 12.50084077282917 16.85696542281429 38.31321160478425 32.32898219957229 5 13.400710029956118 37.68589004299974 35.93941716378589 12.973982763258253 6 32.02710879044972 37.076482751025196 18.12221812097392 12.774190337551167 7 28.119072377254923 31.368217691396993 22.46433960626271 18.048370325085376 8 25.118335431078577 37.282106225391466 20.56373002318512 17.03582832034484 9 26.831247150543064 15.099897523271622 20.20109963107456 37.86775569511076 10 15.336370691673409 28.67391011803483 34.816910521746024 21.172808668545738 11 34.401647563677656 22.034413030094118 25.141383009706946 18.422556396521276 12 23.882871128468725 24.306738790689014 31.901284046638807 19.909106034203454 13 29.38148868077684 21.99127797770035 25.586104502936458 23.041128838586353 14 20.991807238690928 22.291852600367317 27.58248080426621 29.13385935667554 15 23.131951230540245 31.464938530659925 23.04382810546354 22.359282133336286 16 22.174840972154183 27.794610522735297 28.374385101572624 21.656163403537896 17 21.984720123126415 28.679025526545665 27.924783812389364 21.411470537938555 18 21.81988190473206 27.082969460488133 30.260979205444567 20.836169429335243 19 23.954361937443014 26.577804152005857 27.43821727009232 22.029616640458812 20 23.90782452217107 27.31563959994453 28.04944625884376 20.727089619040644 21 22.985339545994087 26.696255446522706 27.097556345245028 23.22084866223818 22 23.43506177302793 28.34125862774972 27.026529753977712 21.19714984524464 23 22.275394422340124 27.973716848896764 27.807489094759035 21.94339963400408 24 23.474552621078875 26.721050968561816 28.45350695717755 21.35088945318176 25 22.934804779832188 27.33460763528998 28.00555438134363 21.725033203534203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1700.0 1 1700.0 2 1589.0 3 1478.0 4 1478.0 5 1478.0 6 2309.5 7 3141.0 8 3141.0 9 3141.0 10 4157.0 11 5173.0 12 5173.0 13 5173.0 14 8069.0 15 10965.0 16 10965.0 17 10965.0 18 18896.0 19 26827.0 20 26827.0 21 26827.0 22 42066.5 23 57306.0 24 57306.0 25 57306.0 26 82869.5 27 108433.0 28 108433.0 29 108433.0 30 138966.0 31 169499.0 32 169499.0 33 169499.0 34 190518.5 35 211538.0 36 211538.0 37 211538.0 38 226957.0 39 242376.0 40 242376.0 41 242376.0 42 254298.0 43 266220.0 44 266220.0 45 266220.0 46 273510.5 47 280801.0 48 280801.0 49 280801.0 50 268366.5 51 255932.0 52 255932.0 53 255932.0 54 226795.0 55 197658.0 56 197658.0 57 197658.0 58 170160.0 59 142662.0 60 142662.0 61 142662.0 62 117280.0 63 91898.0 64 91898.0 65 91898.0 66 71885.5 67 51873.0 68 51873.0 69 51873.0 70 38604.5 71 25336.0 72 25336.0 73 25336.0 74 18336.5 75 11337.0 76 11337.0 77 11337.0 78 8238.0 79 5139.0 80 5139.0 81 5139.0 82 3861.0 83 2583.0 84 2583.0 85 2583.0 86 2177.5 87 1772.0 88 1772.0 89 1772.0 90 1527.5 91 1283.0 92 1283.0 93 1283.0 94 1154.0 95 1025.0 96 1025.0 97 1025.0 98 919.5 99 814.0 100 814.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.7284244901412515 2 0.2132180475259671 3 0.19694045666459287 4 0.1906869189325395 5 0.1652129490534397 6 0.21602294312637343 7 0.4166419513980565 8 0.30200908694210743 9 0.25271649540709845 10 0.22535726782936488 11 0.219655512838375 12 0.22935769270207548 13 0.24453171808132265 14 0.2546017531057322 15 0.33782898321614846 16 0.5056629002896399 17 0.512192329392225 18 0.22190862569771777 19 0.19772214888109954 20 0.19174450251957792 21 0.21045913382064949 22 0.284398021123163 23 0.19556099981193403 24 0.31561972788834125 25 0.3600842204390443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2174769.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.89362016808903 #Duplication Level Percentage of deduplicated Percentage of total 1 86.47369626532044 64.76328162625607 2 9.287218416806283 13.911068170527416 3 2.279595570169657 5.121814943074339 4 0.8049710780712224 2.411487926694531 5 0.3753824864414609 1.4056876678649801 6 0.2134327653331765 0.959085147497268 7 0.12760941322391264 0.66899916467051 8 0.08310939694879188 0.4979490885985404 9 0.05248542873137343 0.3537741387390109 >10 0.2680372544828622 3.5186365838373113 >50 0.01727561484104383 0.8967123349555515 >100 0.013739148683030174 2.134089845287142 >500 0.0020313627007827444 1.0855602729102811 >1k 0.0014157982459995781 2.271853089086998 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4676 0.2150113414344236 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4294 0.19744625751056782 No Hit GTATCAACGCAGAGTACTTTTTTTT 4088 0.18797398712231045 No Hit CTGTAGGACGTGGAATATGGCAAGA 4049 0.18618069321385397 No Hit CTTTAGGACGTGAAATATGGCGAGG 3573 0.16429331115166715 No Hit GTCCTACAGTGTGCATTTCTCATTT 2649 0.12180604008977505 No Hit GGTATCAACGCAGAGTACTTTTTTT 2434 0.11191993264572006 No Hit TATCAACGCAGAGTACTTTTTTTTT 2361 0.10856325430425025 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.59818950886278E-5 0.0 5 0.0 4.59818950886278E-5 0.0 4.59818950886278E-5 0.0 6 0.0 4.59818950886278E-5 0.0 4.59818950886278E-5 0.0 7 0.0 4.59818950886278E-5 0.0 4.59818950886278E-5 0.0 8 0.0 4.59818950886278E-5 0.0 4.59818950886278E-5 0.0 9 0.0 4.59818950886278E-5 0.0 1.3794568526588342E-4 0.0 10 0.0 4.59818950886278E-5 0.0 1.3794568526588342E-4 0.0 11 0.0 4.59818950886278E-5 0.0 1.3794568526588342E-4 0.0 12 0.0 4.59818950886278E-5 0.0 1.3794568526588342E-4 0.0 13 0.0 4.59818950886278E-5 0.0 1.3794568526588342E-4 4.59818950886278E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTC 40 0.005355239 14.219631 14 TAGGACC 905 0.0 13.087531 4 AGGACCT 1820 0.0 11.193434 5 TACACCG 85 5.4675038E-5 11.147497 5 TGTAGGA 2795 0.0 10.645208 2 GTATAAT 255 0.0 10.606671 1 AATCCCG 135 5.999755E-8 10.517496 19 GGACCTG 1825 0.0 10.488051 6 GTAGGAC 2685 0.0 10.48138 3 CTGTAGG 2630 0.0 10.47448 1 GTTTAGA 270 0.0 10.388426 1 GCAGGAC 260 0.0 10.204483 3 GTCCTAA 1330 0.0 10.168049 1 TGGCGAG 2545 0.0 10.114997 18 ATAGGAC 300 0.0 10.107298 3 CACCTTT 650 0.0 10.063124 14 GTATTAG 140 5.129823E-7 10.017411 1 GTCCTAC 2420 0.0 10.017411 1 TAGAAAT 725 0.0 9.80211 4 TAGGACT 300 0.0 9.791219 4 >>END_MODULE