FastQCFastQC Report
Thu 26 May 2016
SRR1512593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174769
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49840.22917376512172097No Hit
GTCCTACAGTGGACATTTCTAAATT49760.22880590996101194No Hit
CTGTAGGACGTGGAATATGGCAAGA48200.22163273432718603No Hit
GTCCTAAAGTGTGTATTTCTCATTT45830.21073502519118123No Hit
CTTTAGGACGTGAAATATGGCGAGG43510.20006722553061956No Hit
GGTATCAACGCAGAGTACTTTTTTT32310.14856750303135643No Hit
TATCAACGCAGAGTACTTTTTTTTT31440.1445670781586458No Hit
GTCCTACAGTGTGCATTTCTCATTT28040.12893323382851238No Hit
GTACATGGGAAGCAGTGGTATCAAC24920.11458688256086048No Hit
CTGAAGGACCTGGAATATGGCGAGA23250.10690790608105964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8800.013.1730694
GGCGAGG11350.012.38357319
GGCCCGT550.003067732612.0916416
TGGACCG752.0698337E-411.4027885
GTCCTAA14100.010.99659251
AGGACCT16800.010.916365
TGTAGGA26150.010.7918182
ATAATAC2050.010.6606533
GACGTGG12750.010.5062777
GGACGTG25900.010.3809386
GTAGGAC26350.010.3132433
AGGACGT26400.010.2581915
AGTCGTC2600.010.2302118
TTAGGAC16350.010.1702153
TGGCGAG27400.010.15540218
GCTTAAC750.002619816210.146551
CTGTAGG26150.010.1125971
GGACCTG16450.010.1069956
TCTACGT951.6412164E-410.0019863
AAGTCGT2700.09.85222157
Produced by FastQC (version 0.11.5)